嗜热嗜酸硫化叶菌中依赖硫的古细菌的环状染色体DNA。

Yamagishi A, Oshima T

Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.

Nucleic Acids Res. 1990 Mar 11;18(5):1133-6. doi: 10.1093/nar/18.5.1133.

The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acidocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acidocaldarius chromosomal DNA. It contained two Notl sites. Both 1.0 and 2.1 Mbp DNA band separated by PFGE were hybridized with the two independent Notl-linking fragment. Each right and left arms of two Notl-linking fragments were hybridized with one of the two DNA bands separated by PFGE. The results indicated that the chromosomal DNA of S.acidocaldarius is circular.

通过脉冲场凝胶电泳（PFGE）分析了嗜硫古细菌嗜酸热硫化叶菌的染色体DNA的形状。用Notl消化的嗜酸热硫化叶菌DNA在约1.0 Mbp和2.1 Mbp处显示出两条DNA带。从嗜酸热硫化叶菌染色体DNA文库中分离出Notl连接克隆。它包含两个Notl位点。通过PFGE分离的1.0和2.1 Mbp DNA带均与两个独立的Notl连接片段杂交。两个Notl连接片段的每个右臂和左臂都与通过PFGE分离的两条DNA带之一杂交。结果表明嗜酸热硫化叶菌的染色体DNA是环状的。