Meirovitch E, Brumberger H, Lis H
Biophys Chem. 1978 Jul;8(3):215-9. doi: 10.1016/0301-4622(78)87003-3.
Mn2+-ESR spectra of soybean, wax bean and lima bean agglutinin at Q- and X-band frequencies show nearly axially symmetric zero field splitting (ZFS); the dominant anisotropic term of the spin hamiltonian is the quadratic ZFS interaction. There is a relatively large distribution of ZFS parameters. No effects of specific inhibitor (N-acetylgalactosamine) on the soybean agglutinin spectrum were observed. The stoichiometric complex obtained on addition of Mn2+ to a Mn2+-free sample of this protein has a spectrum similar to that of the native protein. The small changes in the spectrum are interpreted in terms of a wider distribution of the ZFS parameters at the Mn binding site. Addition of Ca2+ to Mn2+-soybean agglutinin sharpens the lines, possibly because Ca2+ increases the rigidity of the complex.
大豆、黄荚菜豆和利马豆凝集素在Q波段和X波段频率下的Mn2+-ESR光谱显示出近乎轴向对称的零场分裂（ZFS）；自旋哈密顿量的主要各向异性项是二次ZFS相互作用。ZFS参数存在相对较大的分布。未观察到特异性抑制剂（N-乙酰半乳糖胺）对大豆凝集素光谱的影响。向该蛋白质的无Mn2+样品中添加Mn2+得到的化学计量复合物的光谱与天然蛋白质的光谱相似。光谱中的微小变化被解释为Mn结合位点处ZFS参数的分布更宽。向Mn2+-大豆凝集素中添加Ca2+会使谱线变尖锐，可能是因为Ca2+增加了复合物的刚性。
