使用硅光子微环谐振器阵列对捕获剂结合动力学进行多重评估。
Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators.
机构信息
Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
出版信息
Analyst. 2011 Sep 7;136(17):3430-3. doi: 10.1039/c0an00853b. Epub 2010 Nov 18.
Abstract
High affinity capture agents recognizing biomolecular targets are essential in the performance of many proteomic detection methods. Herein, we report the application of a label-free silicon photonic biomolecular analysis platform for simultaneously determining kinetic association and dissociation constants for two representative protein capture agents: a thrombin-binding DNA aptamer and an anti-thrombin monoclonal antibody. The scalability and inherent multiplexing capability of the technology make it an attractive platform for simultaneously evaluating the binding characteristics of multiple capture agents recognizing the same target antigen, and thus a tool complementary to emerging high-throughput capture agent generation strategies.
摘要
高亲和力的生物分子靶标识别捕获试剂是许多蛋白质组学检测方法性能的关键。在此，我们报告了一种无标记的硅光子生物分子分析平台在同时测定两种代表性蛋白质捕获试剂的动力学结合和解离常数方面的应用：凝血酶结合 DNA 适体和抗凝血酶单克隆抗体。该技术的可扩展性和固有多重检测能力使其成为同时评估识别相同靶抗原的多个捕获试剂的结合特性的有吸引力的平台，因此是对新兴的高通量捕获试剂生成策略的补充工具。
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