Block J B, Dietrich M F, Leake R, Laidlaw S A, Vinton N E, Kopple J D
Am J Clin Nutr. 1990 Apr;51(4):698-704. doi: 10.1093/ajcn/51.4.698.
People in developed nations such as the United States and Canada have an increased risk of colon cancer. Fecal mutagens have been detected in the feces of individuals at high risk for colon cancer. We describe a rapid, sensitive, reliable, reproducible high-pressure liquid chromatography (HPLC) method for detecting fecapentaenes, the most active and chief mutagen found in human stool. We found fecapentaene in all the stool samples of adults on typical high-fat, low-fiber Western diets. These fecapentaene concentrations remained largely constant when subjects consumed constant diets. Fecapentaene concentrations were reduced for total-parenteral-nutrition (TPN) patients with severe intestinal malabsorption. This finding with TPN patients may reflect changes in important variables of gut microflora in fecapentaene production. Studies with newborns and children showed that fecapentaenes appeared very early in life but are not present in stool at birth.
在美国和加拿大等发达国家，人们患结肠癌的风险增加。在结肠癌高危个体的粪便中已检测到粪便诱变剂。我们描述了一种快速、灵敏、可靠、可重复的高压液相色谱（HPLC）方法，用于检测粪便戊烯，这是在人类粪便中发现的最具活性和主要的诱变剂。我们在典型的高脂肪、低纤维西方饮食的成年人的所有粪便样本中都发现了粪便戊烯。当受试者食用固定饮食时，这些粪便戊烯浓度基本保持不变。对于患有严重肠道吸收不良的全胃肠外营养（TPN）患者，粪便戊烯浓度降低。TPN患者的这一发现可能反映了粪便戊烯产生中肠道微生物群重要变量的变化。对新生儿和儿童的研究表明，粪便戊烯在生命早期就出现，但出生时粪便中不存在。
