Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Technicka 5, 16628 Prague, Czech Republic.
J Ind Microbiol Biotechnol. 2010 Dec;37(12):1263-70. doi: 10.1007/s10295-010-0801-y. Epub 2010 Nov 18.
Steady-state performance characteristics of a trickle bed reactor (TBR) and a biofilter (BF) in loading experiments with increasing toluene/xylenes inlet concentrations while maintaining a constant loading rate of hydrophilic components (methyl ethyl and methyl isobutyl ketones, acetone, and n-butyl acetate) of 4 g m⁻³ h⁻¹ were evaluated and compared, along with the systems' dynamic responses. At the same combined substrate loading of 55 g m⁻³ h⁻¹ for both reactors, the TBR achieved more than 1.5 times higher overall removal efficiency (RE(W)) than the BF. Increasing the loading rate of aromatics resulted in a gradual decrease of their REs. The degradation rates of acetone and n-butyl acetate were also inhibited at higher loads of aromatics, thus revealing a competition in cell catabolism. A step-drop in loading of aromatics resulted in an immediate increase of RE(W) with variations in the TBR, while the new steady-state value in the BF took 6-7 h to achieve. The TBR consistently showed a greater performance than BF in removing toluene and xylenes. Increasing the loading rate of aromatics resulted in a gradual decrease of their REs. The degradation rates of acetone and n-butyl acetate were also lower at higher OL(AROM), revealing a competition in the cell catabolism. The results obtained are consistent with the proposed hypothesis of greater toxic effects under low water content, i.e., in the biofilter, caused by aromatic hydrocarbons in the presence of polar ketones and esters, which may improve the hydrocarbon partitioning into the aqueous phase.
在提高亲水性成分（甲乙酮、丙酮和乙酸丁酯）负荷率的加载实验中，评估并比较了滴流床反应器（TBR）和生物滤池（BF）的稳态性能特征，同时考察了系统的动态响应。在两个反应器的组合基质负荷率均为 55 g m ⁻³ h ⁻¹ 的情况下，TBR 的总去除效率（RE(W)）比 BF 高 1.5 倍以上。芳香烃负荷率的增加导致其去除率逐渐降低。在较高的芳香烃负荷下，丙酮和乙酸丁酯的降解速率也受到抑制，从而表明细胞分解代谢存在竞争。芳香烃负荷的突然下降导致 RE(W)立即增加，而 BF 则需要 6-7 小时才能达到新的稳态值。TBR 在去除甲苯和二甲苯方面的性能始终优于 BF。芳香烃负荷率的增加导致其去除率逐渐降低。在较高的 OL(AROM)下，丙酮和乙酸丁酯的降解速率也较低，这表明在细胞分解代谢中存在竞争。实验结果与提出的假设一致，即在存在极性酮和酯的情况下，低含水量（即生物滤池）下芳香烃的毒性效应更大，这可能会改善烃类向水相的分配。
