巨大芽孢杆菌萌发基因的鉴定

Identification of germination gene of Bacillus megaterium.

作者信息

Tani K, Kawanishi M, Nishikawa J, Sasaki M, Takubo Y, Nishihara T, Kondo M

机构信息

Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Mar 16;167(2):402-6. doi: 10.1016/0006-291x(90)92036-y.

DOI:10.1016/0006-291x(90)92036-y

PMID:2108667

Abstract

Glucose, KNO3, proline and leucine initiate the spore germination of B. megaterium ATCC 12872, but not of B. megaterium ATCC 19213. In order to isolate the gene concerning germination of B. megaterium ATCC 12872, we constructed its gene library in plasmid vector, and introduced into B. megaterium ATCC 19213. We obtained a transformant whose spores differed from those of the wild type strain with respect to germinability. Spores of this transformant could be germinated by glucose, proline or leucine. The recombinant plasmid prepared from this transformant was found to carry 2 kilobase pairs fragment of B. megaterium ATCC 12872 DNA. This fragment may contain the gene encoding the protein which plays an important role in germination.

摘要

葡萄糖、硝酸钾、脯氨酸和亮氨酸可引发巨大芽孢杆菌ATCC 12872的孢子萌发，但不能引发巨大芽孢杆菌ATCC 19213的孢子萌发。为了分离与巨大芽孢杆菌ATCC 12872萌发相关的基因，我们构建了其在质粒载体中的基因文库，并将其导入巨大芽孢杆菌ATCC 19213。我们获得了一个转化体，其孢子在萌发能力方面与野生型菌株不同。该转化体的孢子可被葡萄糖、脯氨酸或亮氨酸萌发。从该转化体制备的重组质粒被发现携带巨大芽孢杆菌ATCC 12872 DNA的2千碱基对片段。该片段可能包含编码在萌发中起重要作用的蛋白质的基因。