Crespi C L, Steimel D T, Aoyama T, Gelboin H V, Gonzalez F J
Gentest Corporation, Woburn, Massachusetts 01801.
Mol Carcinog. 1990;3(1):5-8. doi: 10.1002/mc.2940030104.
A human lymphoblastoid cell line stably expressing a human cytochrome P450IA2 cDNA was developed. This recombinant cell line displayed P450IA2 protein and estradiol 2-hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000-fold more sensitive to the cytotoxicity and mutagenicity of the carcinogenic mycotoxin aflatoxin B1 than was the parent cell line. The increase in mutagenicity was supported by a corresponding increase in the level of aflatoxin B1 binding to DNA in cells expressing P450IA2 relative to control cells.
构建了一种稳定表达人细胞色素P450IA2 cDNA的人淋巴母细胞系。该重组细胞系表现出P450IA2蛋白和雌二醇2-羟化酶活性，而在亲本细胞系中均未检测到。该重组细胞系对致癌霉菌毒素黄曲霉毒素B1的细胞毒性和致突变性的敏感性也比亲本细胞系高约1000倍。相对于对照细胞，在表达P450IA2的细胞中黄曲霉毒素B1与DNA结合水平的相应增加支持了致突变性的增加。
