Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Univ Paris-Sud 11, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, F-92296 Chatenay-Malabry, France.
Anal Chem. 2010 Dec 15;82(24):10083-9. doi: 10.1021/ac102045x. Epub 2010 Nov 18.
A novel application of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was proposed to efficiently detect and monitor the interaction between polymeric nanoparticles and the β-Amyloid peptide (Aβ(1-42)), a biomarker for Alzheimer's Disease (AD), at concentrations close to physiological conditions. The CE-LIF method allowed the interaction between PEGylated poly(alkyl cyanoacrylate) nanoparticles (NPs) and the soluble Aβ(1-42) peptide monomers to be highlighted. These results were confirmed by surface plasmon resonance (SPR) and confocal laser scanning microscopy (CLSM). Whereas SPR showed an interaction between the NPs and the Aβ(1-42) peptide, CLSM allowed the formation of large aggregates/assemblies at high NP and peptide concentrations to be visualized. All these results suggested that these nanoparticles could bind the Aβ(1-42) peptide and influence its aggregation kinetics. Interestingly, the non-PEGylated poly(alkyl cyanoacrylate) NPs did not alter the aggregation kinetics of the Aβ(1-42) peptide, thus emphasizing the high level of discrimination of the CE-LIF method with respect to NPs.
一种新颖的毛细管电泳与激光诱导荧光检测（CE-LIF）的应用被提出，以有效地检测和监测高分子纳米粒子与β-淀粉样肽（Aβ（1-42））之间在接近生理条件的浓度下的相互作用，Aβ（1-42）是阿尔茨海默病（AD）的生物标志物。CE-LIF 方法能够突出聚乙二醇化聚（烷基氰基丙烯酸酯）纳米颗粒（NPs）与可溶性 Aβ（1-42）肽单体之间的相互作用。这些结果得到了表面等离子体共振（SPR）和共焦激光扫描显微镜（CLSM）的证实。尽管 SPR 显示 NPs 与 Aβ（1-42）肽之间存在相互作用，但 CLSM 允许在高 NP 和肽浓度下可视化大的聚集体/组装的形成。所有这些结果表明，这些纳米颗粒可以结合 Aβ（1-42）肽并影响其聚集动力学。有趣的是，未聚乙二醇化的聚（烷基氰基丙烯酸酯）NPs 不会改变 Aβ（1-42）肽的聚集动力学，从而突出了 CE-LIF 方法对 NPs 的高度区分能力。
