Botany Dept, Tel Aviv Univ., George S. Wise Faculty of Life Sciences. Ramat-Aviv, Tel Aviv 69978, Israel Dept of Botany and Plant Pathology, Michigan State Univ., East Lansing, MI 48824, USA.
Physiol Plant. 1990 Aug;79(4):599-603. doi: 10.1111/j.1399-3054.1990.tb00031.x.
Light Green, an apoplastic probe, was applied to the cut mesocotyl base or to the cut coleoptile apex of etiolated seedlings of Zea mays L. cv. Silver Queen. Probe transport was measured and its tissue distribution determined. In the mesocotyl, there is an apoplastic barrier between cortex and stele. This barrier creates two apoplastic domains which are non-communicating. A kinetic barrier exists between the apoplast of the mesocotyl stele and that of the coleoptile. This kinetic barrier is not absolute and there is limited communication between the apoplasts of the two regions. This kinetic barrier effectively creates two sub-domains. In the coleoptile, there is communication between the apoplast of the vascular strands and that of the surrounding cortical tissue. No apoplastic communication was observed between the coleoptile cortex and the mesocotyl cortex. Thus, the apoplastic space of the coleoptile cortex is a sub-domain of the integrated coleoptile domain and is separate from that of the apoplastic domain of the mesocotyl cortex.
将胞外探针 Light Green 应用于玉米幼苗伸长中胚轴的基部或切去的胚芽鞘顶端。测量探针的运输，并确定其组织分布。在中胚轴中，皮层和中柱之间存在胞外屏障。该屏障形成两个不连通的胞外区域。中胚轴中柱的胞外区和胚芽鞘的胞外区之间存在动力学屏障。该动力学屏障不是绝对的，两个区域的胞外区之间存在有限的通讯。这个动力学屏障有效地创建了两个子区域。在胚芽鞘中，维管束的胞外区与周围皮层组织的胞外区之间存在通讯。在胚芽鞘皮层和中胚轴皮层之间没有观察到胞外通讯。因此，胚芽鞘皮层的胞外空间是整合的胚芽鞘域的子域，与中胚轴皮层的胞外域分开。
