Division of Biochemistry, School of Medicine, Loma Linda University, Loma Linda, CA 92350, United States.
J Colloid Interface Sci. 2011 Feb 15;354(2):662-9. doi: 10.1016/j.jcis.2010.10.028. Epub 2010 Oct 16.
We present a novel approach of using the butylated hydroxytoluene (BHT) antioxidant found in commercial Pluronic F127 samples as a marker of polymer aggregation. The BHT marker was compared to the pyrene dye and static light scattering methods as a way to measure the critical micelle concentration (CMC) and critical micelle temperature (CMT). The n→π(∗) transitions of BHT are sensitive to the microenvironment as demonstrated by plotting the fractional intensities of its excitation (≈280nm) and emission (≈325nm) peaks. BHT is more sensitive to changes in temperature than concentration. The partition coefficient increases ≈40-fold for pyrene compared to ≈2-fold for BHT when the temperature is increased from 25 to 37°C. CMT values determined using the BHT fluorescence decrease with increasing F127 concentration. Our results show that BHT can be used as a reliable marker of changes in the microenvironment of Pluronic F127.
我们提出了一种新的方法，使用商业 Pluronic F127 样品中的抗氧化剂丁基羟基甲苯 (BHT) 作为聚合物聚集的标记物。将 BHT 标记物与芘染料和静态光散射方法进行比较，以测量临界胶束浓度 (CMC) 和临界胶束温度 (CMT)。BHT 的 n→π(∗) 跃迁对微环境敏感，这可以通过绘制其激发（≈280nm）和发射（≈325nm）峰的分强度来证明。与浓度相比，BHT 对温度变化更敏感。当温度从 25°C 升高到 37°C 时，与 BHT 相比，芘的分配系数增加了约 40 倍，而 BHT 增加了约 2 倍。使用 BHT 荧光法测定的 CMT 值随 F127 浓度的增加而降低。我们的结果表明，BHT 可以用作 Pluronic F127 微环境变化的可靠标记物。
