Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, PR China.
Colloids Surf B Biointerfaces. 2011 Jun 15;85(1):86-91. doi: 10.1016/j.colsurfb.2010.10.036. Epub 2010 Oct 30.
In this study, with the aim of designing an ideal anticancer drug carrier, we synthesized novel amphiphilic graft copolymers, P(Glu-alt-PEG)-graft-PCLA, based on poly(ethylene glycol) (PEG) segments and glutamic acid (Glu) units as the hydrophilic main chain, and poly(ɛ-caprolactone-co-lactide) (PCLA) as hydrophobic branches. The chemical structure of the copolymers was characterized by (1)H MNR and FT-IR. The self-assembly of the copolymers to form micelles was studied by TEM, DLS and fluorescence spectroscopy. In vitro doxorubicin controlled release studies demonstrated that these graft copolymer micelles had high drug loading capacity and good controlled released properties, demonstrating their potential as a novel anticancer drug carrier. The drug loaded graft copolymer micelles exhibited efficient inhibition of HeLa cells in in vitro studies.
在这项研究中，我们旨在设计理想的抗癌药物载体，因此合成了新型两亲性接枝共聚物 P(Glu-alt-PEG)-graft-PCLA，其以聚乙二醇（PEG）段和谷氨酸（Glu）单元作为亲水主链，以及聚（己内酯-丙交酯）（PCLA）作为疏水支链。共聚物的化学结构通过 1H NMR 和 FT-IR 进行了表征。通过 TEM、DLS 和荧光光谱研究了共聚物自组装形成胶束的情况。体外阿霉素控制释放研究表明，这些接枝共聚物胶束具有高载药能力和良好的控制释放性能，表明它们有望成为新型抗癌药物载体。载药接枝共聚物胶束在体外研究中对 HeLa 细胞表现出有效的抑制作用。
