Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Innate Immun. 2012 Feb;18(1):44-54. doi: 10.1177/1753425910385962. Epub 2010 Nov 18.
We investigated the impact of two nights of total sleep deprivation (SD) or four nights of rapid eye movement (REM) SD on immunological parameters in healthy men. Thirty-two volunteers were randomly assigned to three protocols (control, total SD or REM SD). Both SD protocols were followed by three nights of sleep recovery. The control and REM SD groups had regular nights of sleep monitored by polysomnography. Circulating white blood cells (WBCs), T- (CD4/CD8) and B-lymphocytes, Ig classes, complement and cytokine levels were assessed daily. Two nights of total SD increased the numbers of leukocytes and neutrophils compared with baseline levels, and these levels returned to baseline after 24 h of sleep recovery. The CD4(+) T-cells increased during the total SD period (one and two nights) and IgA levels decreased during the entire period of REM SD. These levels did not return to baseline after three nights of sleep recovery. Levels of monocytes, eosinophils, basophils and cytokines (IL-1β, IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ) remained unchanged by both protocols of SD. Our findings suggest that both protocols affected the human immune profile, although in different parameters, and that CD4(+) T-cells and IgA levels were not re-established after sleep recovery.
我们研究了两晚完全睡眠剥夺（SD）或四晚快速眼动（REM）SD 对健康男性免疫参数的影响。32 名志愿者被随机分配到三个方案（对照组、完全 SD 组或 REM SD 组）。这两个 SD 方案均随后进行了三晚的睡眠恢复。对照组和 REM SD 组通过多导睡眠图监测有规律的睡眠。每天评估循环白细胞（WBC）、T-（CD4/CD8）和 B 淋巴细胞、Ig 类、补体和细胞因子水平。与基线水平相比，两晚完全 SD 增加了白细胞和中性粒细胞的数量，并且这些水平在 24 小时的睡眠恢复后恢复到基线水平。在完全 SD 期间（第 1 天和第 2 天）CD4(+)T 细胞增加，而在 REM SD 的整个期间 IgA 水平下降。这些水平在三晚睡眠恢复后未恢复到基线。两种 SD 方案均未改变单核细胞、嗜酸性粒细胞、嗜碱性粒细胞和细胞因子（IL-1β、IL-2、IL-4、IL-6、IL-10、TNF-α和 IFN-γ）的水平。我们的发现表明，两种方案均影响了人类免疫谱，尽管在不同的参数中，但 CD4(+)T 细胞和 IgA 水平在睡眠恢复后并未恢复到基线水平。
