Gortzak Y, Kandel R, Deheshi B, Werier J, Turcotte R E, Ferguson P C, Wunder J S
Mount Sinai Hospital, Toronto, Canada.
J Bone Joint Surg Br. 2010 Oct;92(10):1475-9. doi: 10.1302/0301-620x.92b10.23495.
Various chemicals are commonly used as adjuvant treatment to surgery for giant-cell tumour (GCT) of bone. The comparative effect of these solutions on the cells of GCT is not known. In this study we evaluated the cytotoxic effect of sterile water, 95% ethanol, 5% phenol, 3% hydrogen peroxide (H(2)O(2)) and 50% zinc chloride (ZnCI(2)) on GCT monolayer tumour cultures which were established from six patients. The DNA content, the metabolic activity and the viability of the cultured samples of tumour cells were assessed at various times up to 120 hours after their exposure to these solutions. Equal cytotoxicity to the GCT monolayer culture was observed for 95% ethanol, 5% phenol, 3% H(2)O(2) and 50% ZnCI(2). The treated samples showed significant reductions in DNA content and metabolic activity 24 hours after treatment and this was sustained for up to 120 hours. The samples treated with sterile water showed an initial decline in DNA content and viability 24 hours after treatment, but the surviving cells were viable and had proliferated. No multinucleated cell formation was seen in these cultures. These results suggest that the use of chemical adjuvants other than water could help improve local control in the treatment of GCT of bone.
多种化学物质常用于骨巨细胞瘤（GCT）手术的辅助治疗。这些溶液对GCT细胞的比较效果尚不清楚。在本研究中，我们评估了无菌水、95%乙醇、5%苯酚、3%过氧化氢（H₂O₂）和50%氯化锌（ZnCI₂）对从6例患者建立的GCT单层肿瘤培养物的细胞毒性作用。在将肿瘤细胞培养样本暴露于这些溶液后长达120小时的不同时间，评估其DNA含量、代谢活性和活力。观察到95%乙醇、5%苯酚、3% H₂O₂和50% ZnCI₂对GCT单层培养物具有同等的细胞毒性。处理后的样本在处理后24小时显示出DNA含量和代谢活性显著降低，并且这种情况持续长达120小时。用无菌水处理的样本在处理后24小时显示出DNA含量和活力最初下降，但存活细胞仍有活力并已增殖。在这些培养物中未观察到多核细胞形成。这些结果表明，使用除水以外的化学佐剂可能有助于改善骨巨细胞瘤治疗中的局部控制。
