[中国西藏曲拉和云南奶渣中酵母的鉴定及生物多样性]

[Identification and biodiversity of yeasts from Qula in Tibet and milk cake in Yunnan of China].

作者信息

Qing Manjun, Bai Mei, Zhang Yong, Liu Wenjun, Sun Zhihong, Zhang Heping, Sun Tiansong

机构信息

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018, China.

出版信息

Wei Sheng Wu Xue Bao. 2010 Sep;50(9):1141-6.

PMID:21090253

Abstract

OBJECTIVE

To analyze the biodiversity and distribution of the yeast species of Qula in Tibet and milk cake in Yunnan, and to provide essential data for the utilization of yeasts in the traditional dairy products of China.

METHODS

Forty-one yeast strains were isolated from 5 samples of Qula in Tibet and 8 samples of milk cake in Yunnan. The isolates were identified by the large-subunit (26S) rDNA gene D1/D2 domain sequences analysis.

RESULTS

The population of yeast in Qula varied from 10(6) cfu/g to 10(7) cfu/g. The content of yeast ranged from 102 cfu/g to 10(6) cfu/g in milk cake. The average population of yeast in Qula was higher than milk cake for 34 folds. These strains were grouped in 12 species belonging to 10 genera. The dominant species in Qula were Pichia fermentans and Saccharomyces cerevisiae, but Candida zeylanoides and Pichia cactophila were major population in milk cake. The results showed that Pichia was the dominant genera both in Qula and milk cake.

CONCLUSION

There existed that yeasts of great biodiversity both of Qula in Tibet and milk cake in Yunnan, but quite different from each other.

摘要

目的

分析西藏曲拉和云南乳扇中酵母种类的生物多样性及分布情况，为我国传统乳制品中酵母的利用提供基础数据。

方法

从西藏的5份曲拉样品和云南的8份乳扇样品中分离出41株酵母菌株。通过大亚基（26S）rDNA基因D1/D2结构域序列分析对分离菌株进行鉴定。

结果

曲拉中酵母数量为10⁶ cfu/g至10⁷ cfu/g。乳扇中酵母含量为10² cfu/g至10⁶ cfu/g。曲拉中酵母的平均数量比乳扇高34倍。这些菌株分为10个属的12个种。曲拉中的优势种是发酵毕赤酵母和酿酒酵母，而乳扇中的主要种群是锡兰假丝酵母和仙人掌毕赤酵母。结果表明，毕赤酵母属在曲拉和乳扇中均为优势属。