Wu Qiao-Feng, Mao Sen, Cai Wei, Yan Xian-Zhong, Zhao Ji-Lan, Yu Shu-Guang, Tang Yong
College of Acu-moxibustion and Tuina, Chengdu University of Chinese Medicine, Chengdu 610075, China.
Zhen Ci Yan Jiu. 2010 Aug;35(4):287-92.
OBJECTIVE: To compare the effects of electroacupuncture (EA) of Shu-acupoint "Weishu" (BL 21), Mu-acupoint "Zhongwan" (CV 12) and BL 21 + CV 12 on serum large molecular metabolites in functional dyspepsia (FD) rats. METHODS: A total of 50 Wistar rats were randomly divided into control, model, BL 21, CV 12 and BL 21 + CV 12 groups (n = 10/group). FD model was established by clamping the rats' tails for 30 min, once every 3 h, 4 times daily for 7 days. EA was applied to BL 21,CV 12, or BL 21 + CV 12, respectively for 20 min, once daily for 10 days. The rats' venous blood was collected for detecting large molecular metabolites by 1H nuclear magnetic resonance (NMR) spectrometer (Varian UnityInova, 600 MHz). Segmental integral calculus (0.04 ppm per segment) was performed from 6.0-0 ppm (longitudinal eddy-delay, LED) as defaulted 1H spectra values by using the VNMR software. Data was saved as text or excel files after normalization and then used for pattern recognition analyses. All the data were analyzed by principal component analysis (PCA) using the SIMCA-P 10.04 software (Umetrics AB, Umea, Sweden), and orthogonal signal correction and partial least squares discriminant analysis (OSC-PLS-DA). RESULTS: The rat serum proton-LED-1H NMR spectra revealed different metabolic spectra among control,model, BL 21, CV 12, and BL 21 + CV 12 groups. PCA and OSC-PLS-DA showed that compared with the normal control group, FD rats in model group displayed serum N-acetylglucoprotein (NAc) content was reduced considerably, high density lipoprotein (HDL) level decreased to a certain degree, while very low density lipoprotein (VLDL)/LDL increased significantly. In comparison with model group, VLDL/LDL was decreased in BL 21, CV 12 and BL 21 + CV 12 groups, while NAc content increased. Compared with BL 21 and CV 12 groups respectively, HDL level in BL 21 + CV 12 increased to a certain degree. CONCLUSION: EA of BL 21, CV 12 and BL 21 + CV 12 groups can reverse or relieve FD induced increase of VLDL/LDL and decrease of serum NAc level in rats, which may contribute to its function in improving symptoms of FD patients.
目的：比较电针足太阳膀胱经之输穴“胃俞”（BL 21）、任脉之募穴“中脘”（CV 12）以及胃俞（BL 21）与中脘（CV 12）配伍对功能性消化不良（FD）大鼠血清大分子代谢产物的影响。 方法：将50只Wistar大鼠随机分为对照组、模型组、胃俞组（BL 21）、中脘组（CV 12）和胃俞 + 中脘组（BL 21 + CV 12），每组10只。通过夹尾30分钟建立FD模型，每3小时夹尾1次，每天4次，持续7天。分别对胃俞组（BL 21）、中脘组（CV 12）或胃俞 + 中脘组（BL 21 + CV 12）进行电针治疗，每次20分钟，每天1次，共10天。采集大鼠静脉血，用1H核磁共振波谱仪（Varian UnityInova，600 MHz）检测大分子代谢产物。使用VNMR软件，按照默认的1H谱值，从6.0 - 0 ppm（纵向涡流延迟，LED）进行分段积分计算（每段0.04 ppm）。数据经归一化处理后保存为文本或Excel文件，然后用于模式识别分析。所有数据采用SIMCA - P 10.04软件（瑞典于默奥Umetrics AB公司）进行主成分分析（PCA），以及正交信号校正和偏最小二乘判别分析（OSC - PLS - DA）。 结果：大鼠血清质子 - LED - 1H NMR谱显示，对照组、模型组以及胃俞组（BL 21）、中脘组（CV 12）和胃俞 + 中脘组（BL 21 + CV 12）之间存在不同的代谢谱。PCA和OSC - PLS - DA结果显示，与正常对照组相比，模型组FD大鼠血清N - 乙酰葡萄糖蛋白（NAc）含量显著降低，高密度脂蛋白（HDL）水平有一定程度下降，而极低密度脂蛋白（VLDL）/低密度脂蛋白（LDL）显著升高。与模型组相比，胃俞组（BL 21）、中脘组（CV 12）和胃俞 + 中脘组（BL 21 + CV 12）的VLDL/LDL降低，NAc含量增加。与胃俞组（BL 21）和中脘组（CV 12）分别相比，胃俞 + 中脘组（BL 21 + CV 12）的HDL水平有一定程度升高。 结论：胃俞组（BL 21）、中脘组（CV 12）和胃俞 + 中脘组（BL 21 + CV 12）电针可逆转或缓解FD诱导的大鼠VLDL/LDL升高和血清NAc水平降低，这可能是其改善FD患者症状的作用机制。
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