Gao Shana, Li Rui, Tian Huan-huan, Pei En-shi, Cao Bing-yan, Wu Yan
Zhongguo Zhen Jiu. 2014 Nov;34(11):1099-105.
To observe the intervention effects of electroacupuncture at "Yishu" (EX-B 3) on rats with type-2 diabetes mellitus (T2DM), so as to provide experiment references for acupuncture to treat T2DM.
Among seventy male Wistar clean-grade rats, 8 rats were randomly selected into a control group; the rest rats were made T2DM model. Fifty-two rats which were successfully made T2DM model, according to randomized block method, were divided into a model group (10 rats), a medication group (10 rats), an electroacupuncture at "Shenshu" (BL 23) group (11 rats), an electroacupuncture at "Pishu" (BL 20) group (10 rats) and an electroacupuncture at "Yishu" (EX-B 3) group (11 rats). Seven days after successful establishment of model, the rats in the model group were fixed in the self-made rat bag without receiving any treatment; the rats in the medication group, according to body mass (10 mL/kg), were treated with intragastric administration of glimepiride; the rats in all the electroacupuncture groups were treated with electroacupuncture at "Shenshu" (BL 23), "Pishu" (BL 20) and "Yishu" (EX-B 3), respectively. The continuous wave was selected with a frequency of 15 Hz and a current intensity of 4 to 6 mA. The treatment was given 20 min per treatment, once a day, 5 treatments per week for continuous 4 weeks. Before the establishment of model and continuous 4 weeks after the intervention, blood samples were collected from rats' caudal vein, and fasting blood glucose (FBG) was measured with FBG device each week. After the last intervention, the rats were killed and hypothalamus, pituitary and adrenal gland were collected. The colorimetric method was applied to measure the contents of triglyceride (TG), cholesterol (TC), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C); radioimmunoassay was used to test the contents of glycated serum protein (GSP), fasting insulin (FINS), corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH) and cortin (CORT).
Four weeks after the intervention, except that the rat's body mass in the normal group continued to increase, body mass in the model group, medication group and each electroacupuncture group were significantly reduced (P<0.01, P<0.05). Compared with the model group, the FBG in the electroacupuncture at "Pishu" (BL 20) group and electroacupuncture at "Yishu" (EX-B 3) group were obviously reduced (P<0.05, P<0.01); FBG in the electroacupuncture at "Yishu" (EX-B 3) group was lower than that in the medication group and electroacupuncture at "Shenshu" (BL 23) group (both P<0.05). The contents of TG, HDL-C and LDL-C in the electroacupuncture at "Yishu" (EX-B 3) group were reduced (P<0.05, P<0.01), the content of TG was significantly lower than that in the medication group and electroacupuncture at "Shenshu" (BL 23) group (both P<0.05), the content of LDL-C was significantly lower than that in electroacupuncture at "Shenshu" (BL 23) group (P<0.05). Insulin sensitivity index (ISI) in the medication group, electroacupuncture at "Pishu" (BL 20) group and electroacupuncture at "Yishu (EX-B 3)" group were evidently increased (P<0.05, P<0.01); ISI in the medication group was lower than that in the electroacupuncture at "Yishu" (EX-B 3) group (P<0.05). The content of CRH in the electroacupuncture at "Yishu" (EX-B 3) group was lower than that in the medication group and electroacupuncture at "Shenshu" (BL 23) group (P<0.05, P<0.01); the content of CORT in the electroacupuncture at "Yishu" (EX-B 3) group was lower than that in the medication group and electroacupuncture at "Pishu" (BL 20) group (both P<0.05).
Electroacupuncture at "Yishu" (EX-B 3) could reduce the level of CORT to improve the insulin resistance in rats with T2DM, improve insulin sensitivity index, regulate blood lipid metabolism and relieve the hyperactivity of the HPA axis.
观察电针“胰俞”（EX - B 3）对2型糖尿病（T2DM）大鼠的干预作用，为针刺治疗T2DM提供实验参考。
70只雄性Wistar清洁级大鼠，随机选取8只作为对照组；其余大鼠制备T2DM模型。将52只成功制备T2DM模型的大鼠，按随机区组法分为模型组（10只）、药物组（10只）、电针“肾俞”（BL 23）组（11只）、电针“脾俞”（BL 20）组（10只）和电针“胰俞”（EX - B 3）组（11只）。造模成功7天后，模型组大鼠固定于自制鼠袋中不接受任何处理；药物组大鼠按体质量（10 mL/kg）灌胃给予格列美脲；各电针组大鼠分别给予电针“肾俞（BL 23）”、“脾俞（BL 20）”和“胰俞（EX - B 3）”。选用连续波，频率15 Hz，电流强度4～6 mA。每次治疗20 min，每天1次，每周治疗5次，连续治疗4周。造模前及干预后连续4周，每周从大鼠尾静脉采血，用血糖仪测定空腹血糖（FBG）。末次干预后，处死大鼠，取下丘脑、垂体和肾上腺。采用比色法测定甘油三酯（TG）、胆固醇（TC）、高密度脂蛋白（HDL - C）和低密度脂蛋白（LDL - C）含量；采用放射免疫法检测糖化血清蛋白（GSP）、空腹胰岛素（FINS）、促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）和皮质醇（CORT）含量。
干预4周后，除正常组大鼠体质量持续增加外，模型组、药物组及各电针组大鼠体质量均显著降低（P<0.01，P<0.05）。与模型组比较，电针“脾俞”（BL 20）组和电针“胰俞”（EX - B 3）组大鼠FBG明显降低（P<0.05，P<0.01）；电针“胰俞”（EX - B 3）组大鼠FBG低于药物组和电针“肾俞”（BL 23）组（均P<0.05）。电针“胰俞”（EX - B 3）组大鼠TG、HDL - C和LDL - C含量降低（P<0.05，P<0.01），TG含量显著低于药物组和电针“肾俞”（BL 23）组（均P<0.05），LDL - C含量显著低于电针“肾俞”（BL 23）组（P<0.05）。药物组、电针“脾俞”（BL 20）组和电针“胰俞（EX - B 3）”组大鼠胰岛素敏感指数（ISI）明显升高（P<0.05，P<0.01）；药物组ISI低于电针“胰俞”（EX - B 3）组（P<0.05）。电针“胰俞”（EX - B 3）组大鼠CRH含量低于药物组和电针“肾俞”（BL 23）组（P<0.05，P<0.01）；电针“胰俞”（EX - B 3）组大鼠CORT含量低于药物组和电针“脾俞”（BL 20）组（均P<0.05）。
电针“胰俞”（EX - B 3）可降低CORT水平，改善T2DM大鼠胰岛素抵抗，提高胰岛素敏感指数，调节血脂代谢，缓解下丘脑 - 垂体 - 肾上腺（HPA）轴功能亢进。
