di Jeso F, Bartolucci S, Giorgini D
C R Seances Soc Biol Fil. 1978;172(2):274-7.
A specific antibody against cytochrome c1 (pig heart mitochondria) has been obtained. It inhibits the electron transport of the respiratory chain in the intact mitochondria at the cytochrome c1 site of inner mitochondrial membrane ; but it has no effect on the isolated submitochondrial particles (inside-out inner mitochondrial membrane vesicles free of any outer membrane or outside-out inner membrane). Thus the topologic position of cytochrome c1 in the inner mitochondrial membrane is asymetrically lcoated on the outer side of the inner mitochondrial membrane. These results agree with our previous researches on ATP-ase and cytochromes b, c and a, indicating the location on the inner side for the first one, transmembranous for the last one, on the outer side for the others respiratory chain components. Thus the electron transport from cytochrome b to a takes place in the outer region of inner mitochondrial membrane and the transmembranous location of cytochrome-oxidase facilitates the transfer of the electrons to oxygen.
已获得一种针对细胞色素c1（猪心线粒体）的特异性抗体。它在内膜细胞色素c1位点抑制完整线粒体中呼吸链的电子传递；但对分离的亚线粒体颗粒（不含任何外膜的内翻内膜囊泡或外翻内膜）没有影响。因此，细胞色素c1在线粒体内膜中的拓扑位置不对称地位于线粒体内膜外侧。这些结果与我们之前对ATP酶以及细胞色素b、c和a的研究一致，表明第一个位于内侧，最后一个跨膜，其他呼吸链成分位于外侧。因此，电子从细胞色素b传递到a发生在线粒体内膜的外侧区域，细胞色素氧化酶的跨膜位置有利于将电子传递给氧气。
