Institute for Sustainable Agriculture, CSIC, Apdo. 4084, 14080 Córdoba, Spain.
J Agric Food Chem. 2010 Dec 22;58(24):12822-32. doi: 10.1021/jf1036917. Epub 2010 Nov 19.
Responses to Mycosphaerella pinodes in pea were studied by using a proteomics approach. Two-dimensional electrophoresis (2-DE) was used in order to compare the leaf proteome of two pea cultivars displaying different phenotypes (susceptible and partial resistance to the fungus), as well as in response to the inoculation. Multivariate statistical analysis identified 84 differential protein spots under the experimental conditions (cultivars/treatments). All of these 84 protein spots were subjected to MALDI-TOF/TOF mass spectrometry to deduce their possible functions. A total of 31 proteins were identified using a combination of peptide mass fingerprinting (PMF) and MSMS fragmentation. Most of the identified proteins corresponded to enzymes belonging to photosynthesis, metabolism, transcription/translation and defense and stress categories. Results are discussed in terms of responses to pathogens.
采用蛋白质组学方法研究了豌豆中 Mycosphaerella pinodes 的反应。为了比较两个表现出不同表型（对真菌敏感和部分抗性）的豌豆品种的叶片蛋白质组，以及对接种的反应，使用了二维电泳（2-DE）。多变量统计分析确定了实验条件下（品种/处理）84 个差异蛋白点。所有这 84 个蛋白斑点都进行了 MALDI-TOF/TOF 质谱分析，以推断它们可能的功能。通过肽质量指纹图谱（PMF）和 MSMS 片段化的组合，总共鉴定出 31 种蛋白质。鉴定出的大多数蛋白质对应于光合作用、代谢、转录/翻译和防御与应激类别的酶。根据对病原体的反应讨论了结果。
