Desalegn G, Turetschek R, Kaul H-P, Wienkoop S
University of Natural Resources and Life Sciences, Department of Crop Sciences, Austria.
University of Vienna, Department of Ecogenomics and Systems Biology, Austria.
J Proteomics. 2016 Jun 30;143:173-187. doi: 10.1016/j.jprot.2016.03.018. Epub 2016 Mar 22.
The long cultivation of field pea led to an enormous diversity which, however, seems to hold just little resistance against the ascochyta blight disease complex. The potential of below ground microbial symbiosis to prime the immune system of Pisum for an upcoming pathogen attack has hitherto received little attention. This study investigates the effect of beneficial microbes on the leaf proteome and metabolome as well as phenotype characteristics of plants in various symbiont interactions (mycorrhiza, rhizobia, co-inoculation, non-symbiotic) after infestation by Didymella pinodes. In healthy plants, mycorrhiza and rhizobia induced changes in RNA metabolism and protein synthesis. Furthermore, metal handling and ROS dampening was affected in all mycorrhiza treatments. The co-inoculation caused the synthesis of stress related proteins with concomitant adjustment of proteins involved in lipid biosynthesis. The plant's disease infection response included hormonal adjustment, ROS scavenging as well as synthesis of proteins related to secondary metabolism. The regulation of the TCA, amino acid and secondary metabolism including the pisatin pathway, was most pronounced in rhizobia associated plants which had the lowest infection rate and the slowest disease progression.
A most comprehensive study of the Pisum sativum proteome and metabolome infection response to Didymella pinodes is provided. Several distinct patterns of microbial symbioses on the plant metabolism are presented for the first time. Upon D. pinodes infection, rhizobial symbiosis revealed induced systemic resistance e.g. by an enhanced level of proteins involved in pisatin biosynthesis.
长期种植的豌豆产生了巨大的多样性，然而，这种多样性似乎对豌豆壳二孢叶斑病复合体的抗性很低。地下微生物共生作用对豌豆免疫系统进行预激发以应对即将到来的病原体攻击的潜力，迄今为止很少受到关注。本研究调查了在被豌豆壳二孢侵染后，有益微生物在各种共生体相互作用（菌根、根瘤菌、共同接种、非共生）中对植物叶片蛋白质组、代谢组以及表型特征的影响。在健康植物中，菌根和根瘤菌诱导了RNA代谢和蛋白质合成的变化。此外，在所有菌根处理中，金属处理和活性氧清除均受到影响。共同接种导致了与应激相关蛋白质的合成，同时伴随着参与脂质生物合成的蛋白质的调整。植物的疾病感染反应包括激素调节、活性氧清除以及与次生代谢相关的蛋白质合成。三羧酸循环、氨基酸和次生代谢（包括豌豆素途径）的调节在感染率最低且疾病进展最慢的与根瘤菌共生的植物中最为明显。
提供了一项关于豌豆蛋白质组和代谢组对豌豆壳二孢感染反应的最全面研究。首次展示了几种不同的微生物共生对植物代谢的模式。在豌豆壳二孢感染后，根瘤菌共生显示出诱导系统抗性，例如通过提高参与豌豆素生物合成的蛋白质水平。
