[分泌型卷曲相关蛋白基因启动子甲基化状态在急性白血病中的意义]

[The significance of methylation status of secreted frizzled-related protein gene promoter in acute leukemia].

作者信息

Xu Cheng-bo, Shen Jian-zhen, Shen Song-fei, Fu Hai-ying, Zhu Yi-fang, Chen Lu

机构信息

Department of Hematology, Union Hospital, Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, China.

出版信息

Zhonghua Nei Ke Za Zhi. 2010 Sep;49(9):769-71.

PMID:21092449

Abstract

OBJECTIVE

To investigate the relationship between promoter hypermethylation of secreted frizzled-related protein (SFRP) gene and acute leukemia (AL).

METHODS

We examined the promoter methylation status of SFRP1, 2, 4 and 5 in primary or relapsed AL patients, cell lines (HL60, NB4, Molt-4 and Jurkat) and peripheral blood mononuclear cells from healthy people with methylation-specific PCR (MSP).

RESULTS

None of the normal mononuclear cells showed methylation of any SFRP genes. The frequencies of aberrant methylation among the samples were 33.9% (20/59) for SFRP1, 23.7% (14/59) for SFRP2, 6.8% (4/59) for SFRP4 and 10.2% (6/59) for SFRP5 in acute myelocytic leukemia (AML), and 39.3% (11/28) for SFRP1, 28.6% (8/28) for SFRP2, 25.0% (7/28) for SFRP4 and 32.1% (9/28) for SFRP5 in acute lymphoblastic leukemia (ALL). Hypermethylation of SFRP1, 2, 5 genes were present in the 4 AL cell lines. SFRP4 was methylated in NB4, Molt-4 and Jurkat cell lines. However, methylation and unmethylation of SFRP4 were both detected in HL60.

CONCLUSIONS

Hypermethylation of SFRP genes is a common event in the evolution of AL. Methylation of SFRP genes might serve as potential independent biomarkers for early detection of AL.

摘要

目的

探讨分泌型卷曲相关蛋白（SFRP）基因启动子高甲基化与急性白血病（AL）的关系。

方法

采用甲基化特异性PCR（MSP）检测初发或复发AL患者、细胞系（HL60、NB4、Molt-4和Jurkat）以及健康人外周血单个核细胞中SFRP1、2、4和5的启动子甲基化状态。

结果

正常单个核细胞中未检测到任何SFRP基因的甲基化。急性髓细胞白血病（AML）样本中，SFRP1异常甲基化频率为33.9%（20/59），SFRP2为23.7%（14/59），SFRP4为6.8%（4/59），SFRP5为10.2%（6/59）；急性淋巴细胞白血病（ALL）样本中，SFRP1异常甲基化频率为39.3%（11/28），SFRP2为28.6%（8/28），SFRP4为25.0%（7/28），SFRP5为32.1%（9/28）。4种AL细胞系中均存在SFRP1、2、5基因的高甲基化。NB4、Molt-4和Jurkat细胞系中SFRP4发生甲基化，但HL60细胞系中同时检测到SFRP4甲基化与未甲基化情况。