[Cav-1、水通道蛋白1和水通道蛋白5在急性胰腺炎相关性肺损伤大鼠肺组织中的表达及清胰汤的治疗作用]

[Expression of Cav-1, AQP1 and AQP5 in lung of acute pancreatitis-associated lung injury rats and the therapeutic role of Qingyitang].

作者信息

WANG Gang, CHEN Hai-long, REN Feng, LI Jie, LI Ya-qiong

机构信息

Department of General Surgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2010 Sep 28;90(36):2564-9.

PMID:21092464

Abstract

OBJECTIVE

to investigate the expression and location of caveolin-1 (Cav-1), aquaporin 1 (AQP1) and AQP5 in the lung of acute pancreatitis-associated lung injury rats and to determine the role of these molecules in the pathologic progress and the potential therapeutic mechanisms of Qingyitang.

METHODS

forty Wistar rats were randomly divided into sham operation (SHAM) group, acute lung injury (ALI) group, dexamethasone (DEX) group and Qingyitang (QYT) group. ALI was induced by reverse injection of deoxycholate into biliopancreatic duct of rats. Blood and lung tissues were collected after 24 h. Serum amylase, lung wet/dry (W/D) ratio and pathological section were detected to evaluate the degree of lung injury. reverse transcription-polymerase chain reaction was taken to detect the mRNA levels of Cav-1, AQP1 and AQP5. Lipid rafts were prepared for detection of the distribution and expression level of Cav-1, AQP1 and AQP5 proteins by Western blot.

RESULTS

the concentration of serum amylase, the value of W/D and the degree of pathological lung injury obviously increased in ALI rats. Cav-1, AQP1 and AQP5 were present in the lung while the mRNA level decreased in ALI rats. Cav-1 appeared mainly in lipid rafts and less in non-lipid rafts. AQP1 was localized to lipid rafts while AQP5 to non-lipid rafts. The localization of these three molecules in the lung of ALI rats did not change compared with SHAM rats while their protein levels decreased. Compared with ALI rats, the concentration of serum amylase, the value of W/D and the degree of pathological lung injury obviously decreased in DEX and QYT rats. The mRNA and the protein expression of Cav-1, AQP1 and AQP5 increased in various degrees by DEX or QYT treatment.

CONCLUSION

Cav-1 and AQP1 are enriched in lipid rafts while AQP5 in non-lipid rafts. The down-regulated expression of these three molecules may play important role in acute pancreatitis-associated lung injury. DEX and QYT may relieve lung injury effectively by up-regulating the expressions of Cav-1, AQP1 and AQP5.

摘要

目的

探讨小窝蛋白-1（Cav-1）、水通道蛋白1（AQP1）和水通道蛋白5（AQP5）在急性胰腺炎相关性肺损伤大鼠肺组织中的表达及定位，明确这些分子在病理进程中的作用以及清胰汤的潜在治疗机制。

方法

将40只Wistar大鼠随机分为假手术（SHAM）组、急性肺损伤（ALI）组、地塞米松（DEX）组和清胰汤（QYT）组。通过逆行注射脱氧胆酸盐至大鼠胆胰管诱导ALI。24小时后采集血液和肺组织。检测血清淀粉酶、肺湿/干（W/D）比值及病理切片以评估肺损伤程度。采用逆转录-聚合酶链反应检测Cav-1、AQP1和AQP5的mRNA水平。制备脂筏，通过蛋白质免疫印迹法检测Cav-1、AQP1和AQP5蛋白的分布及表达水平。

结果

ALI大鼠血清淀粉酶浓度、W/D值及肺病理损伤程度明显升高。ALI大鼠肺组织中有Cav-1、AQP1和AQP5表达，但其mRNA水平降低。Cav-1主要出现在脂筏中，在非脂筏中较少。AQP1定位于脂筏，而AQP5定位于非脂筏。与SHAM大鼠相比，ALI大鼠肺组织中这三种分子的定位未改变，但其蛋白水平降低。与ALI大鼠相比，DEX组和QYT组大鼠血清淀粉酶浓度、W/D值及肺病理损伤程度明显降低。DEX或QYT处理后，Cav-1、AQP1和AQP5的mRNA及蛋白表达均有不同程度增加。