[水通道蛋白-1、-5表达与新生大鼠高氧诱导肺损伤中肺水肿的关系]

[Relationship between expression of aquaporin-1, -5 and pulmonary edema in hyperoxia-induced lung injury in newborn rats].

作者信息

Yue Dong-Mei, Xue Xin-Dong

机构信息

Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2006 Apr;8(2):147-50.

PMID:16613712

Abstract

OBJECTIVE

Aquaporin (AQP) is a group of cell membrane transporting proteins. The study was designed to investigate the changes of AQP1 and AQP5 in the lung tissue under hyperoxia and their roles in pulmonary edema.

METHODS

Two hundred newborn rats were randomized into different oxygen concentrations exposure: FiO2=0.80 (Experimental group 1), FiO2=0.60 (Experimental group 2), FiO2=0.40 (Experimental group 3) and FiO2=0.21 (Air control group). Rats were sacrificed at 1, 3, 5, 7 and 14 days after the beginning of experiment (10 rats each time point). The expressions of AQP1 and AQP5 were examined by Western Blot. The ratio of lung wet weight to lung dry weight (wet-to-dry weight ratio, W/D), and the protein content in bronchoalveolar lavage fluid (BALF) were measured.

RESULTS

Compared with the Air control group, the W/D ratio and the protein content in BALF in the three experiment groups increased significantly and the increased extent was positively related to the duration and the oxygen concentration of hyperoxia-exposure. The expression of AQP1 in the experimental groups began to decrease at the 3rd day and significant differences were found at the 5th and the 7th days after hyperoxia-exposure compared with that in the Air control group (P < 0.05). The AQP1 expression was restored somewhat at the 14th day after hyperoxia-exposure, but it was still lower in the Experimental groups 1 and 2 than that in the Air control group (P < 0.05). The expression of AQP5 in the experimental groups were reduced compared with that in the Air control group 3 days after hyperoxia-exposure and the decrease of AQP5 expression was associated with duration of hyperoxia-exposure. The comparison among three experimental groups showed that the decrease of AQP1 and AQP5 expressions was associated with the concentration of hyperoxia-exposure.

CONCLUSIONS

The expressions of AQP1 and AQP5 decreased in hyperoxia-induced lung injury and correlated with the severity of pulmonary edema.

摘要

目的

水通道蛋白（AQP）是一类细胞膜转运蛋白。本研究旨在探讨高氧状态下肺组织中AQP1和AQP5的变化及其在肺水肿中的作用。

方法

将200只新生大鼠随机分为不同氧浓度暴露组：FiO2 = 0.80（实验组1）、FiO2 = 0.60（实验组2）、FiO2 = 0.40（实验组3）和FiO2 = 0.21（空气对照组）。在实验开始后的第1、3、5、7和14天处死大鼠（每个时间点10只）。采用蛋白质免疫印迹法检测AQP1和AQP5的表达。测量肺湿重与肺干重之比（湿干重比，W/D）以及支气管肺泡灌洗液（BALF）中的蛋白质含量。

结果

与空气对照组相比，三个实验组的W/D比值和BALF中的蛋白质含量均显著增加，且增加程度与高氧暴露的持续时间和氧浓度呈正相关。实验组中AQP1的表达在第3天开始下降，高氧暴露后第5天和第7天与空气对照组相比差异有统计学意义（P < 0.05）。高氧暴露后第14天，AQP1表达有所恢复，但实验组1和2仍低于空气对照组（P < 0.05）。高氧暴露3天后，实验组中AQP5的表达与空气对照组相比降低，且AQP5表达的降低与高氧暴露持续时间有关。三个实验组之间的比较表明，AQP1和AQP5表达的降低与高氧暴露浓度有关。