RIKILT-Institute of Food Safety, Wageningen UR, Akkermaalsbos 2, 6708 WB Wageningen, the Netherlands.
Int J Food Microbiol. 2011 Mar 1;145 Suppl 1:S68-78. doi: 10.1016/j.ijfoodmicro.2010.10.010. Epub 2010 Oct 21.
A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella 'test' strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.
已经开发出一种针对与致病性相关的 62 个沙门氏菌基因组标记序列的多靶标检测方法，用于对沙门氏菌进行基因分型。该方法基于多重连接检测反应 (LDR)，随后进行定制的 ArrayTube®微阵列检测。该方法的可行性通过使用包含 8 个血清型的 16 个沙门氏菌“测试”菌株与传统 PCR 进行方法比较研究进行了验证。随后，通过分析属于 23 个血清型的 41 个菌株，还测试了 LDR 微阵列检测方法的可行性。除了 4 个血清型外，每个血清型都具有独特的与毒力相关的基因库。LDR 微阵列平台被证明是一种方便、快速和易于使用的工具，具有在食物链中追踪沙门氏菌污染、进行爆发研究以及为支持生物可追溯性模型的风险评估人员提供数据的潜力。
