Department of Urology, Yonsei University College of Medicine, Urological Science Institute, Brain Korea 21 Project for Medical Science, 134, Shinchon-dong, Seudaemun-gu, Seoul 120-752, South Korea.
J Pediatr Urol. 2012 Feb;8(1):67-71. doi: 10.1016/j.jpurol.2010.10.013. Epub 2010 Nov 18.
To evaluate whether the antioxidant vitamin E can prevent the harmful effects of reactive oxidative stress (ROS) that occur during compensatory testicular hypertrophy (CTH).
Thirty Sprague-Dawley rats were divided into six equal groups: neonatal hemicastrated vitamin E (NH_Vit E/NH) and sham surgical controls (NC), and pubertal hemicastrated vitamin E (PH_Vit E/PH) and sham surgical controls (PC). Vitamin E was administered orally to the NH_Vit E and PH_Vit E groups three times a week from week 3-12 prior to sacrifice. Antioxidant enzymes were measured in testis samples from each animal.
Differences in superoxide dismutase activity were observed between the NH (21.04 ± 0.48) and NH_Vit E (22.62 ± 0.64) groups (P = 0.008); the PH (20.59 ± 0.11) and PC (20.91 ± 0.20) groups (P = 0.032); and the PH (20.59 ± 0.11) and PH_Vit E (22.32 ± 1.01) groups (P = 0.008). Thiobarbituric acid-reactive substance in the PH and PH_Vit E groups was 0.097 ± 0.022 and 0.036 ± 0.004 (P = 0.008), respectively; and in the NH and NH_Vit E groups it was 0.135 ± 0.02 and 0.039 ± 0.003 (P = 0.008), respectively.
These results suggest that CTH is not associated with reducing oxidative injury, nor does it prevent ROS-induced cell damage. However, administration of vitamin E does reduce oxidative injury and prevent ROS-induced cell damage in a hemicastrated rat model.
评估抗氧化维生素 E 是否可以预防补偿性睾丸肥大（CTH）过程中发生的活性氧化应激（ROS）的有害影响。
将 30 只 Sprague-Dawley 大鼠分为 6 个相等的组：新生单侧去势维生素 E（NH_Vit E/NH）和假手术对照组（NC），以及青春期单侧去势维生素 E（PH_Vit E/PH）和假手术对照组（PC）。NH_Vit E 和 PH_Vit E 组的大鼠从 3 周到 12 周龄每周口服维生素 E 3 次，直至处死。测量每个动物睾丸样本中的抗氧化酶。
NH（21.04±0.48）和 NH_Vit E（22.62±0.64）组之间的超氧化物歧化酶活性存在差异（P=0.008）；PH（20.59±0.11）和 PC（20.91±0.20）组（P=0.032）；以及 PH（20.59±0.11）和 PH_Vit E（22.32±1.01）组（P=0.008）。PH 和 PH_Vit E 组的硫代巴比妥酸反应物质分别为 0.097±0.022 和 0.036±0.004（P=0.008）；NH 和 NH_Vit E 组的分别为 0.135±0.02 和 0.039±0.003（P=0.008）。
这些结果表明，CTH 不会减少氧化损伤，也不能预防 ROS 诱导的细胞损伤。然而，维生素 E 的给药确实可以减少半去势大鼠模型中的氧化损伤并预防 ROS 诱导的细胞损伤。
