Kaufman David G, Cohen Stephanie M, Chastain Paul D
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Adv Enzyme Regul. 2011;51(1):257-71. doi: 10.1016/j.advenzreg.2010.11.004. Epub 2010 Nov 18.
In summary, recently developed technologies have begun to draw back the curtain of mystery that obscures some of the basic mechanisms of DNA replication at multiple levels. Studies using extended DNA and chromatin fiber techniques have proven valuable for identifying the location of origins of replication at specific genomic sites and determining their temporal order of replication, for identifying and quantifying sites of DNA damage and localizing chromatin proteins in relation to sites of DNA replication. The future potential of these methods include further discoveries in functional genomics and contributions to the elucidation of the histone code. Such studies could prove very valuable in studies of the mechanisms of cancer development, aging, and other processes of disordered genomic functioning.
总之，最近开发的技术已开始揭开笼罩在DNA复制某些基本机制上的多层神秘面纱。使用延伸DNA和染色质纤维技术的研究已证明在确定特定基因组位点的复制起点位置、确定其复制时间顺序、识别和量化DNA损伤位点以及确定与DNA复制位点相关的染色质蛋白定位方面具有重要价值。这些方法未来的潜力包括在功能基因组学方面的进一步发现以及对阐明组蛋白密码的贡献。此类研究在癌症发展、衰老及其他基因组功能紊乱过程的机制研究中可能极具价值。
