Centre de recherche de l'Hôpital Maisonneuve-Rosemont, 5415 boulevard de l'Assomption, Montreal, H1T 2M4, Canada.
Institute for Research in Immunology and Cancer, Université de Montréal, P.O. Box 6128, Succursale Centre-Ville, Montreal, H3C 3J7, Canada; Département de Pathologie et Biologie Cellulaire, Université de Montréal, 2900 Edouard Montpetit Blvd, Montreal, H3T 1J4, Canada.
DNA Repair (Amst). 2021 Aug;104:103140. doi: 10.1016/j.dnarep.2021.103140. Epub 2021 May 23.
A broad spectrum of spontaneous and genotoxin-induced DNA lesions impede replication fork progression. The DNA damage response that acts to promote completion of DNA replication is associated with dynamic changes in chromatin structure that include two distinct processes which operate genome-wide during S-phase. The first, often referred to as histone recycling or parental histone segregation, is characterized by the transfer of parental histones located ahead of replication forks onto nascent DNA. The second, known as de novo chromatin assembly, consists of the deposition of new histone molecules onto nascent DNA. Because these two processes occur at all replication forks, their potential to influence a multitude of DNA repair and DNA damage tolerance mechanisms is considerable. The purpose of this review is to provide a description of parental histone segregation and de novo chromatin assembly, and to illustrate how these processes influence cellular responses to DNA replication roadblocks.
广泛的自发和遗传毒性诱导的 DNA 损伤会阻碍复制叉的前进。作用于促进 DNA 复制完成的 DNA 损伤反应与染色质结构的动态变化有关，这些变化包括在 S 期在整个基因组范围内发生的两个不同过程。第一个过程通常被称为组蛋白回收或亲本组蛋白分离，其特征是将位于复制叉前面的亲本组蛋白转移到新生 DNA 上。第二个过程称为从头组装染色质，由新的组蛋白分子在新生 DNA 上的沉积组成。由于这两个过程发生在所有的复制叉上，它们对多种 DNA 修复和 DNA 损伤耐受机制的影响是相当大的。本综述的目的是描述亲本组蛋白的分离和从头组装染色质，并说明这些过程如何影响细胞对 DNA 复制障碍的反应。
