Center for Perinatal Studies, Swedish Medical Center, University of Washington, Seattle, WA, USA.
Am J Obstet Gynecol. 2011 Feb;204(2):178.e12-21. doi: 10.1016/j.ajog.2010.09.004. Epub 2010 Nov 20.
The role of posttranscription regulation in preeclampsia is largely unknown. We investigated preeclampsia-related placental microRNA (miRNA) expression using microarray and confirmatory quantitative real-time polymerase chain reaction experiments.
Placental expressions of characterized and novel miRNAs (1295 probes) were measured in samples collected from 20 preeclampsia cases and 20 controls. Differential expression was evaluated using Student t test and fold change analyses. In pathway analysis, we examined functions/functional relationships of targets of differentially expressed miRNAs.
Eight miRNAs were differentially expressed (1 up-regulated and 7 down-regulated) among preeclampsia cases compared with controls. These included previously identified candidates (miR-210, miR-1, and a miRNA in the 14q32.31 cluster region) and others that are novel (miR-584 and miR-34c-5p). These miRNAs target genes that participate in organ/system development (cardiovascular and reproductive system), immunologic dysfunction, cell adhesion, cell cycle, and signaling.
Expression of miRNAs that target genes in diverse pathophysiological processes is altered in the setting of preeclampsia.
转录后调控在子痫前期中的作用很大程度上是未知的。我们使用微阵列和确认性实时定量聚合酶链反应实验研究了子痫前期相关的胎盘 microRNA（miRNA）表达。
从 20 例子痫前期病例和 20 例对照中采集样本，测量了 1295 个探针的特征性和新型 miRNA 的胎盘表达。使用学生 t 检验和倍数变化分析评估差异表达。在途径分析中，我们检查了差异表达 miRNA 的靶基因的功能/功能关系。
与对照组相比，子痫前期病例中有 8 个 miRNA 表达差异（1 个上调和 7 个下调）。其中包括先前鉴定的候选物（miR-210、miR-1 和位于 14q32.31 簇区域的 miRNA）和其他新型 miRNA（miR-584 和 miR-34c-5p）。这些 miRNA 的靶基因参与器官/系统发育（心血管和生殖系统）、免疫功能障碍、细胞黏附、细胞周期和信号转导。
在子痫前期中，参与多种病理生理过程的 miRNA 的靶基因表达发生改变。
