Department of Pediatrics, Seoul National University College of Medicine, Seoul 110-799, South Korea.
Diagn Microbiol Infect Dis. 2010 Dec;68(4):345-51. doi: 10.1016/j.diagmicrobio.2010.07.008.
We investigated the genetic structure of Streptococcus pneumoniae isolated from Korean children prior to the use of heptavalent pneumococcal conjugate vaccine (PCV7). A total of 278 pneumococcal isolates were obtained from invasive (n = 77) and noninvasive (n = 201) specimens from children < 5 years of age that visited the Seoul National University Children's Hospital between 1995 and 2005; these samples were serotyped. Two hundred seventy-eight isolates were characterized by multilocus sequence typing, antibiotic susceptibility pattern testing with the E-test. The common serotypes were 19F (22.7%), 23F (19.1%), 19A (14.4%), 6B (11.2%), 6A (8.3%), 14 (5.4%), and 9V (4.3%); these 7 serotypes accounted for 85.3% of the 278 isolates. The isolates belonged to 23 serotypes and 81 sequence types (STs) (including 48 newly discovered STs). The eBURST analysis showed 7 clonal complexes (CCs). CC271 was the main CC of serogroup 19; CC81 and CC3115, serotype 6A; CC81 and CC880, serotype 23F; CC90, serotype 6B; CC166, serotype 9V; and CC554, serotype 14. Nonsusceptibility rates to penicillin and erythromycin among the invasive isolates were 9.1% and 94.8%, respectively. The resultant database may be useful for postvaccine surveillance.
我们研究了在使用 7 价肺炎球菌结合疫苗（PCV7）之前，从韩国儿童中分离出的肺炎链球菌的遗传结构。1995 年至 2005 年间，从首尔国立大学儿童医院就诊的<5 岁儿童的侵袭性（n=77）和非侵袭性（n=201）标本中获得了 278 株肺炎链球菌分离株；对这些样本进行了血清型分型。278 株分离株通过多位点序列分型、E 试验抗生素药敏模式检测进行了特征描述。常见血清型为 19F（22.7%）、23F（19.1%）、19A（14.4%）、6B（11.2%）、6A（8.3%）、14（5.4%）和 9V（4.3%）；这 7 种血清型占 278 株分离株的 85.3%。分离株属于 23 种血清型和 81 种序列型（STs）（包括 48 种新发现的 STs）。eBURST 分析显示了 7 个克隆复合体（CCs）。CC271 是血清群 19 的主要 CC；CC81 和 CC3115 为血清型 6A；CC81 和 CC880 为血清型 23F；CC90 为血清型 6B；CC166 为血清型 9V；CC554 为血清型 14。侵袭性分离株对青霉素和红霉素的不敏感性率分别为 9.1%和 94.8%。该数据库可能对疫苗接种后监测有用。
