豚鼠巨细胞病毒 GP84 是人类巨细胞病毒（HCMV）UL84 基因的功能同源物，可在嵌合 HCMV 中弥补 UL84 的缺失。
Guinea pig cytomegalovirus GP84 is a functional homolog of the human cytomegalovirus (HCMV) UL84 gene that can complement for the loss of UL84 in a chimeric HCMV.
机构信息
Center for Infectious Diseases and Microbiology, Translational Research and Division of Infectious Diseases, University of Minnesota Medical School, Department of Pediatrics, 2001 Sixth Street SE, Minneapolis, MN 55455, USA.
出版信息
Virology. 2011 Feb 5;410(1):76-87. doi: 10.1016/j.virol.2010.10.028. Epub 2010 Nov 20.
Abstract
The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA polymerase accessory protein as described for HCMV pUL84. Additionally, pGP84 exhibited a transdominant inhibitory effect on viral growth as described for HCMV. The inhibitory domain could be localized to a minimal peptide sequence of 99 aa. Knockout of GP84 generated virus with greatly impaired growth kinetics. Lastly, the GP84 ORF was capable of complementing for the loss of the UL84 coding sequence in a chimeric HCMV. Based on this research and previous studies we conclude that GPCMV is similar to HCMV by encoding single copy co-linear functional homologs of HCMV UL82 (pp71), UL83 (pp65) and UL84 genes.
摘要
研究了豚鼠巨细胞病毒（GPCMV）与 HCMV UL84（GP84）共线性基因和潜在的功能同源物。GP84 基因具有延迟的早期转录动力学，并且对 GP84 蛋白（pGP84）的瞬时表达研究表明，它靶向核，并与病毒 DNA 聚合酶辅助蛋白共定位，如 HCMV pUL84 所述。此外，如 HCMV 所述，pGP84 对病毒生长表现出显性抑制作用。抑制结构域可以定位于 99 个氨基酸的最小肽序列。GP84 的敲除产生了生长动力学大大受损的病毒。最后，GP84 ORF 能够在嵌合 HCMV 中弥补 UL84 编码序列的缺失。基于这项研究和以前的研究，我们得出结论，GPCMV 通过编码 HCMV UL82（pp71）、UL83（pp65）和 UL84 基因的单拷贝共线性功能同源物，与 HCMV 相似。
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本文引用的文献
1
Current and new cytomegalovirus antivirals and novel animal model strategies.
Inflamm Allergy Drug Targets. 2010 Sep;9(4):286-99. doi: 10.2174/187152810793358822.
2
Inhibition of human cytomegalovirus replication via peptide aptamers directed against the nonconventional nuclear localization signal of the essential viral replication factor pUL84.
J Virol. 2009 Nov;83(22):11902-13. doi: 10.1128/JVI.01378-09. Epub 2009 Sep 9.
3
Analysis of the association of the human cytomegalovirus DNA polymerase subunit UL44 with the viral DNA replication factor UL84.
J Virol. 2009 Aug;83(15):7581-9. doi: 10.1128/JVI.00663-09. Epub 2009 May 20.
4
The design, structures and therapeutic potential of protein epitope mimetics.
Drug Discov Today. 2008 Nov;13(21-22):944-51. doi: 10.1016/j.drudis.2008.07.008. Epub 2008 Sep 11.
5
Cytomegalovirus UL97 mutations in the era of ganciclovir and maribavir.
Rev Med Virol. 2008 Jul-Aug;18(4):233-46. doi: 10.1002/rmv.574.
6
Expression of the human cytomegalovirus UL97 gene in a chimeric guinea pig cytomegalovirus (GPCMV) results in viable virus with increased susceptibility to ganciclovir and maribavir.
Antiviral Res. 2008 Jun;78(3):250-9. doi: 10.1016/j.antiviral.2008.01.008. Epub 2008 Feb 14.
7
Preconceptual administration of an alphavirus replicon UL83 (pp65 homolog) vaccine induces humoral and cellular immunity and improves pregnancy outcome in the guinea pig model of congenital cytomegalovirus infection.
J Infect Dis. 2007 Mar 15;195(6):789-98. doi: 10.1086/511982. Epub 2007 Feb 6.
8
Human cytomegalovirus UL84 protein contains two nuclear export signals and shuttles between the nucleus and the cytoplasm.
J Virol. 2006 Oct;80(20):10274-80. doi: 10.1128/JVI.00995-06.
9
Pivotal role of animal models in the development of new therapies for cytomegalovirus infections.
Antiviral Res. 2006 Sep;71(2-3):164-71. doi: 10.1016/j.antiviral.2006.05.018. Epub 2006 Jun 19.
10
Antiviral drugs for cytomegalovirus diseases.
Antiviral Res. 2006 Sep;71(2-3):154-63. doi: 10.1016/j.antiviral.2006.05.002. Epub 2006 May 23.
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