人脐带血来源的基质细胞：小鼠半相合移植中造血重建的新资源。
Human umbilical cord blood-derived stromal cells: a new resource in hematopoietic reconstitution in mouse haploidentical transplantation.
作者信息
Zhang C, Chen X-H, Zhang X, Gao L, Kong P Y, Peng X G, Liang X, Gao L, Wang Q Y
机构信息
Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
出版信息
Transplant Proc. 2010 Nov;42(9):3739-44. doi: 10.1016/j.transproceed.2010.08.052.
OBJECTIVE
Our previous study showed that human umbilical cord blood-derived stromal cells (hUcBdSCs) expanded CD34(+) cells in vitro. This study further explored the role of hUcBdSCs in vivo.
METHODS
The cultured hUcBdSCs were infused into transplanted haploidentical mice to observe hematopoietic recovery and complications.
RESULTS
The engraftment was faster in transplantation with hUcBdSCs than without hUcBdSCs. The numbers of fibroblast (CFU-F), granulocyte/monocyte (CFU-GM), erythrocytic (CFU-E), and megakaryocyte (CFU-Mg) colony-forming units were greater among mice transplanted with hUcBdSCs than without hUcBdSCs. The scoring of graft-versus-host disease was significantly lower in mice that had been subjected to transplantation with hUcBdSCs than without hUcBdSCs. The infused hUcBdSCs migrated to the bone marrow of the recipients.
CONCLUSIONS
These data indicated that hUcBdSCs improved hematopoietic reconstitution in haploidentical transplantation in mice.
目的
我们之前的研究表明，人脐带血来源的基质细胞（hUcBdSCs）在体外可扩增CD34(+)细胞。本研究进一步探讨hUcBdSCs在体内的作用。
方法
将培养的hUcBdSCs注入单倍体相合移植小鼠体内，观察造血恢复情况及并发症。
结果
与未注入hUcBdSCs的移植相比，注入hUcBdSCs的移植植入更快。在注入hUcBdSCs的小鼠中，成纤维细胞（CFU-F）、粒细胞/单核细胞（CFU-GM）、红细胞（CFU-E）和巨核细胞（CFU-Mg）集落形成单位的数量比未注入hUcBdSCs的小鼠更多。接受hUcBdSCs移植的小鼠移植物抗宿主病评分显著低于未接受hUcBdSCs移植的小鼠。注入的hUcBdSCs迁移至受体的骨髓。
结论
这些数据表明，hUcBdSCs改善了小鼠单倍体相合移植中的造血重建。
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