Department of Ophthalmology, University Hospital Principe de Asturias, University of Alcalá, Alcalá de Henares, Madrid, Spain.
Am J Ophthalmol. 2011 Jan;151(1):44-52. doi: 10.1016/j.ajo.2010.07.012. Epub 2010 Nov 20.
To evaluate possible changes in the collagen and elastic components of the subepithelial connective tissue of human pterygium.
Immunohistochemical study.
Immunohistochemical staining using antitropoelastin, anti-fibulin-2, and anti-fibulin-3 antibodies was performed in 10 normal conjunctival and 20 pterygium specimens. Masson trichome staining also was performed to study subepithelial connective tissue. Sirius red staining was used to identify collagen type I and III components. Tropoelastin, fibulin-2, and fibulin-3 messenger ribonucleic acid (mRNA) expressions were analyzed in 9 conjunctival and 12 pterygium specimens by quantitative real-time polymerase chain reaction assay.
The subepithelial connective tissue and vessels were more predominant in pterygium compared with the normal conjunctival tissue. Amorphous subepithelial zones were observed in the areas of the pterygium tissue, but not in normal conjunctiva. Increased tropoelastin staining was seen in the pterygium tissue with areas of degenerative changes or immature formation of elastic fibers, as well an increase in tropoelastin mRNA, in contrast with fibulin-2 and fibulin-3 messenger levels. Fibulin-2 and fibulin-3 expression was colocalized in the subepithelial connective tissue and was distributed along blood and lymphatic vessels. Collagen type III, an immature form of collagen, was increased in the pathologic samples in association with a tissue remodeling process.
Elastin metabolism is dysregulated in the pathogenesis of human pterygium with tropoelastin, fibulin-2, and fibulin-3 overexpression in the subepithelial connective tissue.
评估人翼状胬肉的上皮下结缔组织中胶原和弹性成分的可能变化。
免疫组织化学研究。
使用抗原纤维蛋白聚糖-2 和抗原纤维蛋白聚糖-3 抗体对 10 例正常结膜和 20 例翼状胬肉标本进行免疫组织化学染色。还进行了 Masson 三色染色以研究上皮下结缔组织。使用天狼星红染色鉴定 I 型和 III 型胶原成分。通过实时定量聚合酶链反应分析 9 例结膜和 12 例翼状胬肉标本中的原纤维蛋白聚糖-2、原纤维蛋白聚糖-3 信使 RNA（mRNA）表达。
与正常结膜组织相比，翼状胬肉的上皮下结缔组织和血管更明显。在翼状胬肉组织的区域中观察到无定形的上皮下区域，但在正常结膜中没有观察到。在弹性纤维退行性改变或不成熟形成的区域中，可见原纤维蛋白聚糖-2 染色增加，与纤维蛋白聚糖-2 和纤维蛋白聚糖-3 信使水平相比，原纤维蛋白聚糖-2 mRNA 表达增加。纤维蛋白聚糖-2 和纤维蛋白聚糖-3 表达在上皮下结缔组织中存在共定位，并沿血管和淋巴管分布。与组织重塑过程相关，病理性样本中 III 型胶原（一种不成熟的胶原形式）增加。
在人翼状胬肉的发病机制中，弹性蛋白代谢失调，上皮下结缔组织中原纤维蛋白聚糖-2、原纤维蛋白聚糖-3 和原纤维蛋白聚糖-3 表达增加。
