Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
J Chromatogr A. 2010 Dec 24;1217(52):8161-6. doi: 10.1016/j.chroma.2010.10.068. Epub 2010 Oct 29.
Off-line two-dimensional liquid chromatography with tandem mass spectrometry detection (2D-LC/MS-MS) was used to separate a set of metabolomic species. Water-soluble metabolites were extracted from Escherichia coli and Saccharomyces cerevisae cultures and were immediately analyzed using strong cation exchange (SCX)-hydrophilic interaction chromatography (HILIC). Metabolite mixtures are well-suited for multidimensional chromatography as the range of components varies widely with respect to polarity and chemical makeup. Some currently used methods employ two different separations for the detection of positively and negatively ionized metabolites by mass spectrometry. Here we developed a single set of chromatographic conditions for both ionization modes and were able to detect a total of 141 extracted metabolite species, with an overall peak capacity of ca. 2500. We show that a single two-dimensional separation method is sufficient and practical when a pair or more of unidimensional separations are used in metabolomics.
离线二维液相色谱-串联质谱检测（2D-LC/MS-MS）用于分离一组代谢物。从大肠杆菌和酿酒酵母培养物中提取水溶性代谢物，并立即使用强阳离子交换（SCX）-亲水相互作用色谱（HILIC）进行分析。代谢物混合物非常适合多维色谱法，因为其组成成分在极性和化学组成方面差异很大。目前一些使用的方法采用两种不同的分离方法，用于通过质谱法检测正离子和负离子化的代谢物。在这里，我们开发了一种适用于两种离子化模式的单一色谱条件，并能够检测到总共 141 种提取的代谢物，整体峰容量约为 2500。当在代谢组学中使用一对或多对一维分离时，我们表明，单一的二维分离方法是足够且实用的。
