CNR, Institute of Science of Food Production (ISPA), Prov.le Lecce-Monteroni, 73100 Lecce, Italy.
Syst Appl Microbiol. 2010 Dec;33(8):461-7. doi: 10.1016/j.syapm.2010.09.003. Epub 2010 Nov 20.
The diversity of indigenous Oenococcus oeni strains was investigated by molecular and biochemical characterization of isolates from Malvasia Nera wine, an economically important red wine of the Salento Region (Apulia, Italy), during spontaneous malolactic fermentation (MLF). A total of 82 isolates of this species, identified by species-specific PCR and 16S rDNA sequence analysis, was molecularly characterized by the amplified fragment length polymorphism (AFLP) technique. Three main groups resulted from cluster analysis and showed intraspecific homology higher than 50%, and a total of seven subgroups, with similarity values ranged from 80% to 98%, were obtained within these groups. Enzymatic activities, such as esterase, β-glucosidase, protease, and the consumption rate of l-malic acid, citric acid, acetaldehyde and arginine were assessed in the representative strains, according to AFLP analysis. The results showed different enzymatic activities and consumption rates of the tested metabolites among the strains. No correlation between molecular and biochemical data was observed. The evidence of biochemical variability observed among Malvasia Nera strains demonstrated that the wine aroma can be modulated depending on the strains involved in MLF. Hence, the heterogeneity existing within natural O. oeni populations represents an interesting ecological source that can be useful for technological purposes.
采用分子和生化特征分析的方法，对马尔瓦西亚·内拉（Malvasia Nera）葡萄酒自然发生的苹果酸-乳酸发酵（MLF）过程中的本土片球菌（Oenococcus oeni）菌株多样性进行了研究。马尔瓦西亚·内拉葡萄酒是意大利普利亚大区（阿普利亚）萨伦托地区（Salento Region）一种具有重要经济价值的红葡萄酒。研究共分离到 82 株该属菌株，通过种特异性 PCR 和 16S rDNA 序列分析对其进行鉴定，随后采用扩增片段长度多态性（AFLP）技术对其进行分子特征分析。聚类分析结果显示有 3 个主要群组，组内菌株的种内同源性大于 50%，且每个群组内都可分为 7 个亚组，其相似度值在 80%到 98%之间。根据 AFLP 分析，对代表性菌株的酯酶、β-葡萄糖苷酶、蛋白酶活性以及 L-苹果酸、柠檬酸、乙醛和精氨酸的消耗率等酶学特性进行了评估。结果表明，不同菌株之间的酶学特性和代谢物消耗率存在差异。分子和生化数据之间未观察到相关性。马尔瓦西亚·内拉菌株之间存在的生化可变性表明，葡萄酒的香气可以根据参与 MLF 的菌株进行调节。因此，天然 O. oeni 种群内的异质性代表了一种有趣的生态来源，可用于技术目的。
