TIFAC CORE in NDDS, Pharmacy Department, M.S. University of Baroda, Fatehgunj, Vadodara-390 002, Gujarat, India.
J Control Release. 2011 Feb 28;150(1):2-22. doi: 10.1016/j.jconrel.2010.11.002. Epub 2010 Nov 21.
A great deal of effort has been made over the years to develop liposomes that have targeting vectors (oligosaccharides, peptides, proteins and vitamins) attached to the bilayer surface. Most studies have focused on antibody conjugates since procedures for producing highly specific monoclonal antibodies are well established. Antibody conjugated liposomes have recently attracted a great deal of interest, principally because of their potential use as targeted drug delivery systems and in diagnostic applications. A number of methods have been reported for coupling antibodies to the surface of stealth liposomes. The objective of this review is to enumerate various strategies which are employed in the modification and conjugation of antibodies to the surface of stealth liposomes. This review also describes various derivatization techniques of lipids prior and after their use in the preparation of liposomes. The use of single chain variable fragments and affibodies as targeting ligands in the preparation of immunoliposomes is also discussed.
多年来，人们付出了大量努力来开发具有靶向载体（寡糖、肽、蛋白质和维生素）的脂质体，这些靶向载体附着在双层表面。由于生产高度特异性单克隆抗体的程序已经成熟，因此大多数研究都集中在抗体缀合物上。抗体偶联脂质体最近引起了极大的兴趣，主要是因为它们可能被用作靶向药物传递系统和在诊断应用中。已经报道了许多将抗体连接到隐形脂质体表面的方法。本综述的目的是列举用于修饰和连接抗体到隐形脂质体表面的各种策略。本综述还描述了脂质在用于制备脂质体之前和之后的各种衍生化技术。还讨论了使用单链可变片段和亲和体作为免疫脂质体制备中的靶向配体。
