Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Florida College of Medicine, 653-1 West 8th Street-L14, Jacksonville, FL 32209, United States.
Neurosci Lett. 2011 Jan 25;488(3):272-4. doi: 10.1016/j.neulet.2010.11.044. Epub 2010 Nov 21.
The human and mouse homologs of the rat thyroid hormone responsive protein (THRP), c-abl-interacting protein 2 (Abi-2), are critically involved in neurological development. The Abi-2 gene is evolutionarily conserved in vertebrates, and is also found in Xenopus laevis and Drosophila melanogaster. The THRP gene is one of the few genes regulated by thyroid hormone in adult animals. Sequence analysis of the 5'-flanking region of the THRP gene identified a putative thyroid hormone response element (TRE) that is conserved between rat and human. To determine whether or not THRP regulates neural growth and development, THRP was constitutively expressed in transgenic X. laevis. Growth of most animals was halted in early neurulation while the few animals that survived the process developed into grossly malformed tadpoles. In contrast, control animals reached late embryonic stage 25. These observations suggest that THRP over-expression in early development is not compatible with completion of early embryogenesis and that a different strategy needs to be employed to investigate THRP function in this model.
大鼠甲状腺激素反应蛋白（THRP）、c-abl 相互作用蛋白 2（Abi-2）的人类和鼠类同源物在神经发育中起着至关重要的作用。Abi-2 基因在脊椎动物中是进化保守的，也存在于非洲爪蟾和黑腹果蝇中。THRP 基因是成年动物中少数受甲状腺激素调节的基因之一。对 THRP 基因 5'侧翼区的序列分析鉴定出一个推定的甲状腺激素反应元件（TRE），在大鼠和人类之间是保守的。为了确定 THRP 是否调节神经生长和发育，THRP 在转基因非洲爪蟾中持续表达。大多数动物的生长在早期神经胚形成时停止，而少数幸存下来的动物发育成严重畸形的蝌蚪。相比之下，对照动物达到晚期胚胎期 25。这些观察结果表明，THRP 在早期发育中的过表达与早期胚胎发生的完成不兼容，需要采用不同的策略来研究该模型中 THRP 的功能。
