Schreiber W E, Bowling S
Department of Pathology, Vancouver General Hospital, BC, Canada.
Ann Clin Biochem. 1990 Mar;27 ( Pt 2):129-32. doi: 10.1177/000456329002700207.
We have developed a rapid, automated assay for glycogen phosphorylase that measures the degradative reaction. The substrate contains higher concentrations of phosphate and AMP than other methods, and the enzyme is preincubated with the substrate before adding phosphate to start the reaction. These modifications improve the activity and linearity of the assay. The new assay compares favourably with a standard phosphorylase assay in the synthetic direction and is linear to 500 U/L. We have used this assay to measure phosphorylase activity in human tissue samples and muscle biopsy specimens.
我们已经开发出一种用于糖原磷酸化酶的快速自动化检测方法，该方法可测量降解反应。与其他方法相比，该检测方法的底物含有更高浓度的磷酸盐和AMP，并且在添加磷酸盐启动反应之前，先将酶与底物进行预孵育。这些改进提高了检测方法的活性和线性。新的检测方法在合成方向上与标准磷酸化酶检测方法相比具有优势，并且在500 U/L范围内呈线性。我们已经使用该检测方法来测量人体组织样本和肌肉活检标本中的磷酸化酶活性。
