大鼠单倍体生殖细胞中存在一种独特的Gsα亚基信使核糖核酸。

A unique mRNA species for the alpha subunit of Gs is present in rat haploid germ cells.

作者信息

Haugen T B, Paulssen E J, Gordeladze J O, Hansson V

机构信息

Institute of Medical Biochemistry, University of Oslo, Norway.

出版信息

Biochem Biophys Res Commun. 1990 Apr 16;168(1):91-8. doi: 10.1016/0006-291x(90)91679-m.

DOI:10.1016/0006-291x(90)91679-m

PMID:2109612

Abstract

The 1.9 kb mRNA encoding the alpha subunit of Gs observed in the somatic cells of the rat testis was not seen in the germ cells (pachytene spermatocytes and round spermatids) as revealed by Northern blot analysis. Interestingly, the cDNA probe for Gs alpha detected a mRNA of shorter chain length (0.9 kb) exclusively in the haploid germ cells (round and elongated spermatids). However, no ADP ribosylated protein was detected in membranes from germ cells after cholera toxin treatment. The 0.9 kb transcript may represent a haploid germ cell-specific variant of Gs alpha, which is functionally different from the stimulatory component of the adenylate cyclase system.

摘要

通过Northern印迹分析发现，在大鼠睾丸体细胞中观察到的编码Gsα亚基的1.9 kb mRNA在生殖细胞（粗线期精母细胞和圆形精子细胞）中未出现。有趣的是，Gsα的cDNA探针仅在单倍体生殖细胞（圆形和延长型精子细胞）中检测到较短链长度（0.9 kb）的mRNA。然而，霍乱毒素处理后，在生殖细胞膜中未检测到ADP核糖基化蛋白。0.9 kb转录本可能代表Gsα的单倍体生殖细胞特异性变体，其在功能上不同于腺苷酸环化酶系统的刺激成分。