Zhang Xiaoxiao, Ohta Aaron T, Garmire David
University of Hawaii at Manoa (UHM), Honolulu, HI 96822, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010:6518-21. doi: 10.1109/IEMBS.2010.5627084.
A microfluidic device was designed having the ability to continuously produce monodisperse microcapsules with controlled cell loading. The design included stages of inertial focusing, droplet generation, and photopolymerization. Prototype microfluidic devices were fabricated in polydimethylsiloxane (PDMS) to demonstrate each stage using poly(ethylene-glycol)-diacrylate (PEGDA) as the encapsulating material and oil as the droplet-containing medium, creating a water-in-oil emulsion. 10.3-µm-diameter fluorescent polystyrene beads were used as cell simulants. In the first stage, inertial focusing was demonstrated using a straight-channel configuration. In the second stage, droplets with a 60±5µm diameter were generated. In the third stage, successful encapsulation of the beads in hydrogel droplets was verified. This technology can significantly impact a wide research area ranging from cellular therapeutics to single-cell manipulation.
设计了一种微流控装置，该装置能够连续生产具有可控细胞负载的单分散微胶囊。该设计包括惯性聚焦、液滴生成和光聚合阶段。使用聚二甲基硅氧烷（PDMS）制造了微流控装置原型，以聚（乙二醇）-二丙烯酸酯（PEGDA）作为封装材料，油作为含液滴介质，展示每个阶段，形成油包水乳液。使用直径为10.3 µm的荧光聚苯乙烯珠作为细胞模拟物。在第一阶段，使用直通道配置展示了惯性聚焦。在第二阶段，生成了直径为60±5 µm的液滴。在第三阶段，验证了珠子在水凝胶液滴中的成功封装。这项技术可以对从细胞治疗到单细胞操作的广泛研究领域产生重大影响。
