可溶性钙结合蛋白：来自鳗鱼骨骼肌的小清蛋白和钙调蛋白。
Soluble calcium-binding proteins: parvalbumins and calmodulin from eel skeletal muscle.
作者信息
Dubois I, Gerday C
机构信息
Institut de Chimie (B6) au Sart Tilman, Université de Liège, Belgium.
出版信息
Comp Biochem Physiol B. 1990;95(2):381-5. doi: 10.1016/0305-0491(90)90091-7.
PMID:2109671
Abstract
- Eel skeletal muscle contains three parvalbumin isoforms. The overall parvalbumin concentration in the muscle is 0.5 mmol kg-1 wet weight. 2. Calmodulin (0.1 mumol kg-1 wet weight) was purified by extraction with ethylenediamine tetraacetate-containing buffer, fractionation with trichloroacetic acid and separation by ion exchange chromatography on DEAE-cellulose and by molecular sieving on Ultrogel AcA 54. 3. Troponin-C-free calmodulin was obtained by fast protein liquid chromatography on a Mono Q column.
摘要
- 鳗鱼骨骼肌含有三种小清蛋白亚型。肌肉中小清蛋白的总浓度为0.5毫摩尔/千克湿重。2. 通过用含乙二胺四乙酸的缓冲液提取、用三氯乙酸分级分离以及在DEAE-纤维素上进行离子交换色谱分离和在Ultrogel AcA 54上进行分子筛分离，纯化了钙调蛋白（0.1微摩尔/千克湿重）。3. 通过在Mono Q柱上进行快速蛋白质液相色谱法获得了无肌钙蛋白C的钙调蛋白。
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