Bursuker I, Pearce M T
Pharmaceutical Research and Development Division, Bristol-Myers Company, Wallingford, CT 06492.
J Interferon Res. 1990 Feb;10(1):1-11. doi: 10.1089/jir.1990.10.1.
The state of active immunity to Meth A fibrosarcoma in mice immunized with an admixture of Meth A cells and Propionibacterium acnes is associated with possession by the host of spleen cells capable of producing interferon-gamma (IFN-gamma) upon in vitro restimulation with irradiated tumor cells. The ability of spleen cells from immunized mice to produce IFN-gamma in response to irradiated Meth A cells decays as active antitumor immunity is replaced by a state of immunological memory. The IFN-producing cells are L3T4+Ly2+, cyclophosphamide-sensitive and radiosensitive T cells, as determined by their sensitivity to corresponding monoclonal antibodies and complement. The induction of IFN-gamma production by in vivo tumor-sensitized T cells is tumor specific, in that spleen cells from mice immunized against Meth A fibrosarcoma can produce IFN in response to irradiated Meth A cells but not in response to another syngeneic tumor M109 lung carcinoma.
用甲基胆蒽A（Meth A）细胞与痤疮丙酸杆菌的混合物免疫的小鼠，对Meth A纤维肉瘤的主动免疫状态与宿主拥有在体外经照射的肿瘤细胞再刺激后能够产生γ干扰素（IFN-γ）的脾细胞有关。随着主动抗肿瘤免疫被免疫记忆状态所取代，免疫小鼠的脾细胞对经照射的Meth A细胞产生IFN-γ的能力逐渐衰退。通过它们对相应单克隆抗体和补体的敏感性确定，产生IFN的细胞是L3T4 + Ly2 +、对环磷酰胺敏感且对辐射敏感的T细胞。体内肿瘤致敏T细胞诱导IFN-γ产生具有肿瘤特异性，即免疫抵抗Meth A纤维肉瘤的小鼠的脾细胞能够对经照射的Meth A细胞产生IFN，但对另一种同基因肿瘤M109肺癌无反应。
