Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran.
Pharmacol Rep. 2010 Sep-Oct;62(5):858-63. doi: 10.1016/s1734-1140(10)70345-5.
The present study investigated the effects of subcutaneous (sc) and intracerebroventricular (icv) injections of physostigmine (a cholinesterase inhibitor), atropine (an antagonist of muscarinic cholinergic receptors) and hexamethonium (an antagonist of nicotinic cholinergic receptors) on the acute corneal nociception in rats. Local application of 5 M NaCl solution on the corneal surface of the eye produced a significant nociceptive behavior, characterized by eye wiping. The number of eye wipes was counted during the first 30 s. The sc (0.25, 0.5 and 1 mg/kg) and icv (1.25, 2.5, 5 and 10 μg) injections of physostigmine significantly (p < 0.05) decreased the number of eye wipes. Atropine and hexamethonium at (2 mg/kg, sc and 20 μg, icv) had no effects when used alone, however, atropine, but not hexamethonium prevented the antinociception induced by physostigmine (sc and icv). The results of this study indicate that the central muscarinic, but not nicotinic receptors might be involved in the antinociceptive effect of physostigmine in the acute corneal model of pain in rats.
本研究探讨了皮下（sc）和脑室内（icv）注射毒扁豆碱（一种乙酰胆碱酯酶抑制剂）、阿托品（毒蕈碱型胆碱能受体拮抗剂）和六烃季铵（烟碱型胆碱能受体拮抗剂）对大鼠急性角膜伤害感受的影响。局部应用 5 M NaCl 溶液于眼球角膜表面可产生显著的伤害性行为，表现为眨眼。在最初的 30 秒内记录眨眼次数。毒扁豆碱（sc，0.25、0.5 和 1 mg/kg；icv，1.25、2.5、5 和 10 μg）的 sc 和 icv 注射显著（p < 0.05）减少了眨眼次数。阿托品（sc，2 mg/kg；icv，20 μg）和六烃季铵（icv，20 μg）单独使用时没有效果，但阿托品而非六烃季铵可预防毒扁豆碱（sc 和 icv）引起的镇痛作用。本研究结果表明，中枢毒蕈碱受体，而不是烟碱型胆碱能受体，可能参与了毒扁豆碱在大鼠急性角膜疼痛模型中的镇痛作用。
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