Department of Pathology, United Arab Emirates University, Al Ain, United Arab Emirates.
Islets. 2009 Sep-Oct;1(2):102-5. doi: 10.4161/isl.1.2.9273.
Growth factors such as insulin growth factor-1 (IGF-1) and epidermal growth factor (EGF) play important roles in the regulation of cell metabolism in response to different internal as well as external stimuli. It is not clear how Type 2 diabetes mellitus influences the expression of these growth factors in the endocrine pancreas. The localization of IGF-1 and EGF was analyzed to determine the impact of Type 2 diabetes on the pattern of distribution of these growth factors in pancreatic islet cells.
Pancreatic tissue samples retrieved, during pancreatectomy for pancreatic tumour, from patients with and without Type 2 diabetes were paraffin embedded. The tissue samples were chosen from non-neoplastic, clear excisional, margins. The expression of IGF-1 and EGF was investigated using immunofluorescence techniques.
IGF-1- and EGF-immunoreactive cells were observed in many cells located in the central region of pancreatic islets. The percentage of islet cells expressing IGF-1 and EGF decreased significantly (P < 0.05) in diabetic patients compared to control.
The number of human pancreatic islet cells expressing IGF-1 and EGF decreased significantly after the onset of Type 2 diabetes. These growth factors may play a role in the function of the endocrine pancreas and in the pathogenesis of the diabetes mellitus.
生长因子，如胰岛素样生长因子-1（IGF-1）和表皮生长因子（EGF），在调节细胞代谢方面发挥着重要作用，以应对不同的内部和外部刺激。目前尚不清楚 2 型糖尿病如何影响这些生长因子在内分泌胰腺中的表达。本研究分析了 IGF-1 和 EGF 的定位，以确定 2 型糖尿病对胰岛细胞中这些生长因子分布模式的影响。
从因胰腺肿瘤而行胰腺切除术的患者中获取胰腺组织样本，这些患者分为 2 型糖尿病组和非 2 型糖尿病组。组织样本取自非肿瘤、明确切除的切缘。使用免疫荧光技术检测 IGF-1 和 EGF 的表达。
在胰岛中央区域的许多细胞中观察到 IGF-1 和 EGF 免疫反应性细胞。与对照组相比，糖尿病患者的胰岛细胞表达 IGF-1 和 EGF 的比例显著下降（P<0.05）。
2 型糖尿病发病后，人胰岛细胞表达 IGF-1 和 EGF 的数量显著减少。这些生长因子可能在胰岛的功能和糖尿病的发病机制中发挥作用。
