Cell and Molecular Therapy Center (NUCEL), University of São Paulo, São Paulo, Brazil.
Islets. 2009 Nov-Dec;1(3):224-31. doi: 10.4161/isl.1.3.9695.
Transplantation of pancreatic islets isolated from organ donors constitutes a promising alternative treatment for type1 Diabetes, however, it is severely limited by the shortage of organ donors. Ex-vivo islet cell cultures appear as an attractive but still elusive approach for curing type 1 Diabetes. It has recently been shown that, even in the absence of fibrotic overgrowth, several factors, such as insufficient nutrition of the islet core, represent a major barrier for long-term survival of islets grafts. The use of immobilized dispersed cells may contribute to solve this problem due to conceivably easier nutritional and oxygen support to the cells. Therefore, we set out to establish an immobilization method for primary cultures of human pancreatic cells by adsorption onto microcarriers (MCs). Dispersed human islets cells were seeded onto Cytodex1 microcarriers and cultured in bioreactors for up to eight days. The cell number increased and islet cells maintained their insulin secretion levels throughout the time period studied. Moreover, the cells also presented a tendency to cluster upon five days culturing. Therefore, this procedure represents a useful tool for controlled studies on islet cells physiology and, also, for biotechnological applications.
从器官捐献者中分离出的胰岛移植为 1 型糖尿病提供了一种很有前途的治疗方法，但它受到器官捐献者短缺的严重限制。离体胰岛细胞培养似乎是一种有吸引力但仍难以实现的治疗 1 型糖尿病的方法。最近的研究表明，即使没有纤维化过度生长，一些因素，如胰岛核心的营养不足，也代表了胰岛移植物长期存活的主要障碍。由于可以更轻松地为细胞提供营养和氧气支持，固定化分散细胞的使用可能有助于解决这个问题。因此，我们着手通过吸附到微载体（MC）上来建立人胰腺细胞原代培养的固定化方法。将分散的人胰岛细胞接种到 Cytodex1 微载体上，并在生物反应器中培养长达 8 天。细胞数量增加，并且在整个研究期间胰岛细胞保持其胰岛素分泌水平。此外，细胞在培养五天后也呈现出聚集的趋势。因此，该方法代表了对胰岛细胞生理学进行控制研究的有用工具，也代表了生物技术应用的有用工具。
