Department of Integrative Biosciences, Oregon National Primate Center, Oregon Health and Science University, Portland, USA.
Islets. 2010 Jan-Feb;2(1):24-9. doi: 10.4161/isl.2.1.10041.
AIMS/ HYPOTHESIS: Caspase-3 is a main effector caspase of the apoptotic cascade. Involvement of caspase-3 has been implicated in a β cell cloned cell line from type 1 diabetic subjects and in isolated islets from type 2 diabetic subjects.
The control islets revealed cleaved caspase-3 positive cells in about 4.7 % in the total islet cells with large and small islets positive at 4.1 % and 6.8%, respectively. The islets from type 1 diabetic patients showed higher immunopositive cells at 16 % in the total islets with large and small islets positive at 14% and 17%, respectively, 3.4, 3.6 and 2.4 times that of the corresponding control values.
Using commercially available rabbit anti-cleaved caspase-3 antibody, immunocytochemical staining was performed on 8 cases of pancreatic tissues from type 1 diabetic subjects and age-matched controls obtained at autopsy. Islets were divided into large islets containing more than 34 islet cells and small islets containing less than 34 islet cells. Cleaved caspase-3 immunostained islet cells were calculated for large and small islets, respectively, with total number of islet cells and total percentage of cleaved caspase-3 positive cells.
CONCLUSION/INTERPRETATION: The islets from type 1 diabetics were a mixture of major small-sized islets consisting of insulin-poor and glucagon-rich cells with more caspase-3 positive cells, and occasional large islets, consisting of non-insulin-cells (> 1%) but glucagon- , somatostatin- and PP-rich cells with normal caspase-3 positive cells. The more positive staining for caspase-3 in islets from type 1 diabetics may correspond to accelerated apoptosis cascade in the islets before processing to eventual cell death.
目的/假设：Caspase-3 是凋亡级联的主要效应 Caspase。Caspase-3 的参与已被牵连到来自 1 型糖尿病患者的克隆β细胞系和来自 2 型糖尿病患者的分离胰岛中。
对照胰岛中总胰岛细胞中 cleaved caspase-3 阳性细胞约为 4.7%，大胰岛和小胰岛阳性率分别为 4.1%和 6.8%。1 型糖尿病患者的胰岛显示出更高的免疫阳性细胞，总胰岛中的阳性细胞为 16%，大胰岛和小胰岛中的阳性细胞分别为 14%和 17%，分别为相应对照值的 3.4、3.6 和 2.4 倍。
使用市售的兔抗 cleaved caspase-3 抗体，对 8 例 1 型糖尿病患者和尸检获得的年龄匹配对照者的胰腺组织进行免疫细胞化学染色。胰岛分为含有大于 34 个胰岛细胞的大胰岛和含有小于 34 个胰岛细胞的小胰岛。分别计算大胰岛和小胰岛的 cleaved caspase-3 免疫染色胰岛细胞，总胰岛细胞数和 cleaved caspase-3 阳性细胞的总百分比。
结论/解释：1 型糖尿病患者的胰岛是由富含胰岛素和胰高血糖素的小胰岛和偶尔的大胰岛组成的混合物，大胰岛由非胰岛素细胞（> 1%）组成，但富含胰高血糖素、生长抑素和 PP，cleaved caspase-3 阳性细胞正常。1 型糖尿病患者胰岛中 caspase-3 的阳性染色较多，可能对应于胰岛在最终细胞死亡之前的凋亡级联的加速。
