鱼腥藻属CA（ATCC 33047）中氨对固氮酶从氧失活状态恢复的调控
Control of nitrogenase recovery from oxygen inactivation by ammonia in the cyanobacterium Anabaena sp. strain CA (ATCC 33047).
作者信息
Smith R L, Van Baalen C, Tabita F R
机构信息
University of Texas Marine Science Institute, Port Aransas 78373.
出版信息
J Bacteriol. 1990 May;172(5):2788-90. doi: 10.1128/jb.172.5.2788-2790.1990.
The control of nitrogenase recovery from inactivation by oxygen was studied in Anabaena sp. strain CA (ATCC 33047). Nitrogenase activity (acetylene reduction) in cultures grown in 1% CO2 in air was inhibited by exposure to 1% CO2-99% O2 and allowed to recover in the presence of high oxygen tensions. Cultures exposed to hyperbaric levels of oxygen in the presence of 10 mM NH4NO3 were incapable of regaining nitrogenase activity, whereas control cultures returned to 65 to 80% of their original activity within about 3 h after exposure to high oxygen tension. In contrast to the regulation of heterocyst differentiation and nitrogenase synthesis, recovery from oxygen inactivation in this organism was shown to be under the control of NH4+ rather than NO3-.
在鱼腥藻属CA菌株（ATCC 33047）中研究了固氮酶从氧失活中恢复的调控。在空气中1% CO₂ 条件下培养的培养物中的固氮酶活性（乙炔还原）通过暴露于1% CO₂ - 99% O₂ 而受到抑制，并在高氧张力存在下恢复。在10 mM NH₄NO₃ 存在下暴露于高压氧水平的培养物无法恢复固氮酶活性，而对照培养物在暴露于高氧张力后约3小时内恢复到其原始活性的65%至80%。与异形胞分化和固氮酶合成的调控相反，该生物体中氧失活后的恢复显示受NH₄⁺ 而非NO₃⁻ 的控制。
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