Misono H, Yoshimura T, Nagasaki S, Soda K
Department of Agricultural Chemistry, Kochi University.
J Biochem. 1990 Jan;107(1):169-72. doi: 10.1093/oxfordjournals.jbchem.a123003.
The stereochemical aspects of the L-lysine epsilon-dehydrogenase reaction were examined with (6R)-L-[6-3H]lysine and (6S)-DL-[6-3H]lysine. When (6S)-DL-[6-3H]lysine was used as a substrate, the tritium was found in the product, delta 1-piperideine-6-carboxylate. In contrast, the radioactivity from (6R)-L-[6-3H]lysine was not retained in the product. Thus, the pro-R hydrogen at the prochiral C-6 carbon of L-lysine is specifically abstracted by the enzyme: the enzyme behaves stereochemically as an amino acid D-dehydrogenase.
用(6R)-L-[6-³H]赖氨酸和(6S)-DL-[6-³H]赖氨酸研究了L-赖氨酸ε-脱氢酶反应的立体化学方面。当使用(6S)-DL-[6-³H]赖氨酸作为底物时，在产物δ¹-哌啶-6-羧酸盐中发现了氚。相反，(6R)-L-[6-³H]赖氨酸的放射性没有保留在产物中。因此，L-赖氨酸前手性C-6碳上的前-R氢被该酶特异性提取：该酶在立体化学上表现为氨基酸D-脱氢酶。
