从冷冻组织样本中高效且可扩展地进行 DNA 和 RNA 的串联提取。
Efficient and scalable serial extraction of DNA and RNA from frozen tissue samples.
机构信息
Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.
出版信息
Chem Commun (Camb). 2011 Jan 7;47(1):547-9. doi: 10.1039/c0cc02248a. Epub 2010 Nov 23.
PMID:21103565
Abstract
Advances in cancer genomics have created a demand for scalable sample processing. We here present a process for serial extraction of nucleic acids from the same frozen tissue sample based on magnetic silica particles. The process is automation friendly with high recoveries of pure DNA and RNA suitable for analysis.
摘要
癌症基因组学的进展催生了对可扩展样本处理的需求。我们在此介绍了一种基于磁性硅基粒子从同一冷冻组织样本中连续提取核酸的方法。该方法易于自动化操作，可实现高纯度 DNA 和 RNA 的高回收率，适用于分析。
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