College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, 434023, China.
Biol Trace Elem Res. 2011 Nov;143(2):1142-8. doi: 10.1007/s12011-010-8902-z. Epub 2010 Nov 23.
The effect of cerium on mitochondria isolated from hybrid rice Shanyou 63 (Oryza sativa L) was investigated. Through in vivo culture, low dose Ce3+ promoted, but higher dose Ce3+, restrained mitochondrial heat production. However, through vitro incubation, Ce3+ showed only inhibitory action on mitochondrial energy turnover, the concentration required for 50% inhibition being 46.7 μM. In addition, Ce3+, like Ca2+, induced rice mitochondrial swelling and decreased membrane potential (△ψ), which was inhibited by the specific permeability transition inhibitor cyclosporine A (CsA). The induction approached a constant level while mitochondrial metabolism was fully prevented by Ce3+. These results demonstrated that cerium influenced rice mitochondria in vivo and in vitro via different action pathways, and the latter involved the opening of rice mitochondrial permeability.
研究了铈对杂交水稻汕优 63（Oryza sativa L）分离的线粒体的影响。通过体内培养，低剂量的 Ce3+ 促进了线粒体的产热，而高剂量的 Ce3+ 则抑制了线粒体的产热。然而，通过体外孵育，Ce3+ 仅对线粒体能量转换表现出抑制作用，其 50%抑制浓度为 46.7 μM。此外，Ce3+ 像 Ca2+ 一样，诱导水稻线粒体肿胀并降低膜电位（△ψ），而特异性通透性转换抑制剂环孢菌素 A（CsA）可以抑制这种作用。当 Ce3+ 完全阻止线粒体代谢时，诱导作用接近一个恒定水平。这些结果表明，铈通过不同的作用途径影响体内和体外的水稻线粒体，而后者涉及到水稻线粒体通透性的开放。
