雌激素诱导未成熟和成熟大鼠子宫中C-jun原癌基因的表达。

Estrogen induced expression of the C-jun proto-oncogene in the immature and mature rat uterus.

作者信息

Webb D K, Moulton B C, Khan S A

机构信息

Department of Anatomy and Cell Biology, University of Cincinnati, College of Medicine, OH 45267.

出版信息

Biochem Biophys Res Commun. 1990 Apr 30;168(2):721-6. doi: 10.1016/0006-291x(90)92381-9.

DOI:10.1016/0006-291x(90)92381-9

PMID:2110455

Abstract

The expression of the proto-oncogene c-jun in response to estradiol treatment in immature and mature rat uterine tissue was measured using a cDNA encoding the mouse c-jun proto-oncogene. This probe hybridized to a major RNA band of 2.7 kb and a minor 3.2 kb band. In Northern blots of total RNA from both immature and mature rat uteri, estradiol treatment resulted in at least a 3 fold increase in expression of the 2.7 kb band over control levels by 3 hr post injection. By 12 hr post injection, expression of c-jun mRNA had returned to control levels. A strong induction (greater than 5 fold) of c-jun mRNA expression was also observed in stroma-myometrial tissue isolated from mature rats approximately 3 hours after treatment with estradiol. The similar kinetics of induction of c-fos and c-jun emphasizes the functional significance of the fos/jun heterodimer in control of uterine cell proliferation.

摘要

使用编码小鼠原癌基因c-jun的cDNA，检测未成熟和成熟大鼠子宫组织中原癌基因c-jun在雌二醇处理后的表达情况。该探针与一条2.7 kb的主要RNA条带和一条3.2 kb的次要条带杂交。在未成熟和成熟大鼠子宫总RNA的Northern印迹中，注射后3小时，雌二醇处理使2.7 kb条带的表达量比对照水平至少增加3倍。注射后12小时，c-jun mRNA的表达已恢复到对照水平。在用雌二醇处理约3小时后，从成熟大鼠分离的基质-肌层组织中也观察到c-jun mRNA表达的强烈诱导（大于5倍）。c-fos和c-jun诱导的相似动力学强调了fos/jun异二聚体在控制子宫细胞增殖中的功能意义。