Gabrilevskaia O V, Ivanov A A, Terekhov S M, Shilov E M
Biull Eksp Biol Med. 1990 Jan;109(1):57-60.
The optimal method to obtain primary rat glomerular cell culture was developed. The method includes isolation of rat glomeruli by pressing slices of renal cortex through sieves, treatment of glomeruli with collagenase and cultivation on gelatin-coated plastic wells. Using electron microscopy and immunofluorescent staining with antibodies to intermediate filaments, factor VIII, myosin, common leucocyte antigen two cell types were identified; epithelial cells and contractile mesangial cells. Further subcultivation allowed to obtain a pure mesangial cell culture.
开发了获取原代大鼠肾小球细胞培养物的最佳方法。该方法包括通过筛网挤压肾皮质切片来分离大鼠肾小球，用胶原酶处理肾小球，并在明胶包被的塑料孔中培养。使用电子显微镜和针对中间丝、因子VIII、肌球蛋白、普通白细胞抗原的抗体进行免疫荧光染色，鉴定出两种细胞类型；上皮细胞和收缩性系膜细胞。进一步传代培养可获得纯系膜细胞培养物。
